Investigation of GPNMB function in endoplasmic reticulum stress and oxidative stress responses

• Project/Area Number: 25870605
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology; Pharmacology in pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: Tsuruma Kazuhiro 岐阜薬科大学, 薬学部, 講師 (50524980)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 小胞体ストレス / 神経変性疾患 / GPNMB / 小胞体ストレス応答機構 / GRP78 / スプライシング / 発現調節

Outline of Final Research Achievements

Endoplasmic reticulum (ER) stress is known to be associated with Alzheimer's disease, amyotrophic lateral sclerosis and glaucoma. Excessive ER stress causes cell death. In this study, I revealed that glycoprotein non-metastatic melanoma B (GPNMB), which is related to ALS and glaucoma, was upregulated by ER stress and GPNMB reduced the cell death induced by ER stress. GPNMB promoted the mRNA maturation of glucose-regulated protein 78 (GRP78/BiP) known as a molecular chaperone in ER.

Research Products

• [Presentation] 未定 (2016)
  • Author(s): 野田泰裕、鶴間一寛、嶋澤雅光、原英彰
  • Organizer: 第11回小胞体ストレス研究会
  • Place of Presentation: 岐阜大学サテライトキャンパス
  • Year and Date: 2016-10-10
• [Presentation] 小胞体ストレス存在下におけるGPNMBのBiP pre-mRNAスプライシング促進作用 (2016)
  • Author(s): 野田泰裕, 鶴間一寛, 髙田真史, 田中彦孝, 嶋澤雅光, 原英彰
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-03-09