| Project/Area Number |
25870620
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
Anesthesiology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Takeshita Jun 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (40433263)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | micro RNA / 肺傷害 / 急性肺障害 / 遺伝子治療 |
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| Outline of Final Research Achievements |
We focused on microRNA expressions in macrophages and monocytes in the presence of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). In the first step, we prepared a macrophage (and THP-1 cell) inflammatory model by lipopolysaccharide (LPS) exposure, and analyzed microRNA expressions. In the second step, we investigated the changes in intracellular signaling molecules (e.g., TNF-α) with the transgenesis of microRNA using Nucleofector. The results of the first step showed the altered expression of miR-21 based on comprehensive analysis and the real-time PCR method. MiR-21 transgenesis induced the increased expressions of IL-10 and P-Akt (P308) proteins based on Western blotting.
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