Investigation of kisspeptin-regulating mechanism by suckling stimulus

• Project/Area Number: 25870621
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology); Obstetrics and gynecology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YAMADA Shunji 京都府立医科大学, 医学(系)研究科(研究院), 講師 (40454079)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: キスペプチン / 授乳期 / 吸乳刺激 / 生殖内分泌 / 神経回路 / カルシトニン遺伝子関連ペプチド / 脚周囲核 / 神経経路

Outline of Final Research Achievements

Kisspeptin is the head of neural peptide regulating reproductive functions, and the expression is remarkably suppressed by suckling stimulus from pups in lactating mother rats who are inhibited reproductive function. The present project was undertaken to investigate what kind of mechanism does suckling stimulus suppress kisspeptin expression through. The present study demonstrated that calcitonin gene-related peptide (CGRP) in the thalamus and brainstem were increased during lactation and thalamic CGRP neurons were activated by suckling stimulus.

Research Products

• [Journal Article] Estrogen-Related Receptor beta Reduces the Subnuclear Mobility of Estrogen Receptor alpha and Suppresses Estrogen-Dependent Cellular Function. (2015)
  • Author(s): Tanida T, Matsuda K I, Yamada S, Hashimoto T, Kawata M
  • Journal Title: J Biol Chem Volume: - Issue: 19 Pages: 12332-12345
  • DOI: 10.1074/jbc.m114.619098
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats (2014)
  • Author(s): Shunji Yamada, Mitsuhiro Kawata
  • Journal Title: Frontiers in Neuroscience Volume: 8 Pages: 1-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distribution of Gastrin-Releasing peptide in the rat trigeminal and spinal somatosensory systems (2014)
  • Author(s): K Takanami, et al.
  • Journal Title: Journal of Comparative Neurology Volume: 522 Issue: 8 Pages: 1858-1873
  • DOI: 10.1002/cne.23506
  • Peer Reviewed
• [Presentation] 授乳期の視床下部弓状核におけるプロダイノルフィン遺伝子発現 (2015)
  • Author(s): 山田俊児、畑幸一、石上真由子、笹倉康照、小澤孝明、河田光博
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場 (兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] ロードーシスに関与する中脳中心灰白質の神経細胞の特性 (2014)
  • Author(s): 山田俊児
  • Organizer: 第20回「性と生殖」公開シンポジウム
  • Place of Presentation: 早稲田大学所沢キャンパス（埼玉県所沢市）
  • Year and Date: 2014-12-20
  • Invited
• [Presentation] Involvement of brain stem calcitonin gene-related peptide in suckling stimulus during lactation (2014)
  • Author(s): Shunji Yamada, Kouichi Hata, Yasuteru Sasakura, Mitsuhiro Kawata
  • Organizer: 8th International Congress of Neuroendocrinology
  • Place of Presentation: Hilton Sydney (Australia)
  • Year and Date: 2014-08-17 – 2014-08-20
• [Presentation] 授乳期の視床下部弓状核におけるプロダイノルフィン遺伝子発現 (2014)
  • Author(s): 山田俊児、畑幸一、笹倉康照、河田光博
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 栃木
• [Presentation] エストロゲン関連受容体ERRβによるエストロゲン応答性調節機構 (2014)
  • Author(s): 谷田任司, 松田賢一, 山田俊児, 高浪景子、河田光博
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 栃木
• [Presentation] ラット三叉神経節および脊髄後根神経節におけるgastrin-releasing peptideの発現と回路 (2014)
  • Author(s): 高浪景子、坂本浩隆、松田賢一、佐藤慧太、谷田任司、山田俊児、井上海平、越智拓海、坂本竜哉、河田光博
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 栃木
• [Presentation] The role of androgen receptor during postnatal periods in masculinization of nervous system. (2013)
  • Author(s): Shunji Yamada, Miku Ohoya, Kenichi Matsuda, Mitsuhiro Kawata
  • Organizer: The 36th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kyoto
• [Presentation] The co-localization of nuclear estrogen-related receptor (ERR) γ and estrogen receptor (ER) α in the rat brain. (2013)
  • Author(s): Takashi Tanida, Ken-ichi Matsuda, Shunji Yamada, Keiko Takanami, Mitsuhiro Kawata
  • Organizer: The 36th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kyoto
• [Presentation] Distribution of gastrin-releasing peptide in the trigeminal sensory system. (2013)
  • Author(s): Keiko Takanami, Hirotaka Sakamoto, Ken-Ichi Matsuda, Keita Satoh, Kaihei Inoue, Shunji Yamada, Takashi Tanida, Takumi Oti, Tatsuya Sakamoto, and Mitsuhiro Kawata
  • Organizer: The 36th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kyoto
• [Presentation] 吸乳刺激で活性化する脳幹の神経細胞とカルシトニン遺伝子関連ペプチド（CGRP）神経の関係 (2013)
  • Author(s): 山田俊児、笹倉康照、畑幸一、河田光博
  • Organizer: 第40回日本神経内分泌学会学術集会
  • Place of Presentation: 宮崎
• [Presentation] エストロゲン関連受容体ERRβはエストロゲン受容体ERαの可動性を減ずることでその転写活性を抑制する (2013)
  • Author(s): 谷田任司, 松田賢一, 山田俊児, 高浪景子、河田光博
  • Organizer: 第40回日本神経内分泌学会学術集会
  • Place of Presentation: 宮崎