| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The aim of this study is to identify anti-allergic compounds from Japanese pepper (Zanthoxylum piperitum, ZP) and to elucidate the mechanism. Using famous rat mast cell line RBL-2H3, we screened the Japanese pepper extract on the basis of an inhibitory effect on a release of chemical mediator (degranulation) of IgE sensitized mast cell by antigen stimulation. As a result of this study, two inhibitory compounds were isolated from ZP. These two compounds attenuated intracellular Ca2+ mobilization induced by antigen stimulation. In addition, those inhibited the phosphorylation of Fyn related with migration of granule to the cell membrane, and the phosphorylation of Lyn which is upstream signal of intracellular Ca2+ mobilization. Furthermore, these compounds prevented membrane ruffling which is caused while degranulation.
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