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The role of the MTA in the palate cleft graft by tooth-derived stem cells

Research Project

Project/Area Number 25870663
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Surgical dentistry
Research InstitutionOhu University

Principal Investigator

MAEDA Toyonobu  奥羽大学, 歯学部, 助教 (10382756)

Co-Investigator(Renkei-kenkyūsha) KATO Yasumasa   (50214408)
KIMURA Yuichi   (60211877)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsMTA / 骨形成 / 小胞体ストレス / オステオカルシン / コラーゲン / MMP / 骨芽細胞 / 石灰化 / ATF6 / 骨 / 再生 / 糖代謝
Outline of Final Research Achievements

To understand effect of MTA in the palate cleft graft, we determined whether MTA induces osteoblastic differentiation.
MTA enhanced mineralization concomitantly with alkaline phosphatase activity in a dose and time dependent fashions. MTA increased production of collagens and MMPs.MTA also induced osteocalcin mRNA. Interestingly, we observed induction of Atf6 mRNA expression and activation of Atf6 by the MTA treatment. Forced expression of p50 Atf6 markedly enhanced Ocn mRNA expression. Chromatin immunoprecipitation assay investigated increase in p50Atf6 binding to Ocn promoter region by MTA treatment.Furthermore, knockdown of Atf6 gene expression abrogated MTA induced mineralization. These results suggest that MTA induces in vitro osteoblastic differentiation through the Atf6 - osteocalcin axis as the ER stress signaling.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] Mineral trioxide aggregate induces osteoblastogenesis via Atf62015

    • Author(s)
      Maeda T, Suzuki A, Yuzawa S, Baba Y, Kimura Y, Kato Y
    • Journal Title

      Bone Reports

      Volume: 2 Pages: 36-43

    • DOI

      10.1016/j.bonr.2015.03.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Deguelin Induced Apoptosis by Targeting both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines2015

    • Author(s)
      Baba Y, Fujii M, Maeda T, Suzuki A, Yuzawa S, Kato Y
    • Journal Title

      Biomed Res Int.

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CRISPR/Cas9を用いたMC3T3-E1細胞におけるGpr81遺伝子のノックアウト2015

    • Author(s)
      前田豊信,鈴木厚子,湯澤仁,馬場優,加藤靖正
    • Journal Title

      奥羽大学歯学誌

      Volume: 41 Pages: 123-128

    • NAID

      120005759844

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acidic extracellular pH promotes epithelial mesenchymal transition in Lewis lung carcinoma model2014

    • Author(s)
      Suzuki A, Maeda T, Baba Y, Shimamura K, Kato Y
    • Journal Title

      Cancer Cell International

      Volume: 14 Issue: 1 Pages: 129-129

    • DOI

      10.1186/s12935-014-0129-1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acidic extracellular microenviromental and cancer2013

    • Author(s)
      Kato Y, Ozawa S, Miyamoto C, Maehata Y, Suzuki A, Maeda T, Baba Y
    • Journal Title

      Cancer Cell International

      Volume: 13(1) Issue: 1 Pages: 89-89

    • DOI

      10.1186/1475-2867-13-89

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 小胞体ストレスセンサーATF6を介した石灰化の一例2015

    • Author(s)
      前田豊信,鈴木厚子,湯澤仁,阿部匡聡,加藤靖正
    • Organizer
      東北骨代謝研究会
    • Place of Presentation
      仙台
    • Year and Date
      2015-02-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] MTAによるATF6を介した骨芽細胞の石灰化促進作用2014

    • Author(s)
      前田豊信, 原元信貴, 有馬英夫, 木村裕一
    • Organizer
      日本歯科保存学会2014年度秋季学術大会(141回)
    • Place of Presentation
      山形
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] MTAは骨芽細胞様細胞でATF6を介して石灰化を促進させる。2014

    • Author(s)
      前田豊信, 鈴木厚子, 湯澤仁, 阿部匡聡, 加藤靖正
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] MC3T3-E1細胞において、 MTAはATF6を介して石灰化を促進させる.2014

    • Author(s)
      前田豊信,鈴木厚子,湯澤仁,阿部匡聡,加藤靖正
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Mineral Trioxide Aggregate セメントの骨形成作用2014

    • Author(s)
      前田豊信,鈴木厚子,湯澤仁,馬場優,木村裕一,加藤靖正
    • Organizer
      奥羽大学歯学会
    • Place of Presentation
      郡山
    • Year and Date
      2014-06-14
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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