Establishment of a rapid method for measuring sphingosine-1-phosphate transporter activity

• Project/Area Number: 25870695
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy; Biological pharmacy
• Research Institution: Setsunan University (2015-2016); Teikyo Heisei University (2013-2014)
• Principal Investigator: Kobayashi Naoki 摂南大学, 薬学部, 助教 (90532250)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 蛍光プレートリーダー / スフィンゴシン1リン酸 / 赤血球 / S1P / NBD-S1P / 脂質メディエーター

Outline of Final Research Achievements

Sphingosine-1-phosphate (S1P) is a signal transmitter released from erythrocytes, and essential for migration of lymphocytes to the bloodstream. An S1P transporter, which exports the S1P from erythrocytes to blood plasma, can be considered a potential target for immune-suppressive drugs. In this study, a rapid method to measure the activity of erythrocyte S1P transporter was established. This method is useful for screening of drugs that possess the ability to suppress the activity of the S1P transporter in erythrocytes.

Research Products

• [Journal Article] Fluorescence-based rapid measurement of sphingosine-1-phosphate transport activity in erythrocytes (2016)
  • Author(s): Naoki Kobayashi, Masato Otsuka, Akihito Yamaguchi and Tsuyoshi Nishi
  • Journal Title: Journal of Lipid Research Volume: 57 Issue: 11 Pages: 2088-2094
  • DOI: 10.1194/jlr.d071068
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Update Review 新しい創薬標的としての脂質メディエーター分泌輸送体 (2016)
  • Author(s): 西 毅、久野 悠、小林 直木、山口 明人
  • Journal Title: 実験医学 Volume: 34 Pages: 2356-2361
• [Journal Article] Molecular and physiological functions of sphingosine 1-phosphate transporters (2014)
  • Author(s): Nishi, T., Kobayashi, N., Hisano, Y., Kawahara, A. and Yamaguchi, A
  • Journal Title: Biochimica et Biophysica Acta Volume: 1841 Issue: 5 Pages: 759-765
  • DOI: 10.1016/j.bbalip.2013.07.012
  • Peer Reviewed
• [Journal Article] β-Lactam Selectivity of Multidrug Transporters AcrB and AcrD Resides in the Proximal Binding Pocket (2014)
  • Author(s): Kobayashi,N., Tamura,N., van Veen, HW., Murakami,S. and Yamaguchi,A.
  • Journal Title: Journal of Biological Chemistry Volume: 289 Issue: 15 Pages: 10680-10690
  • DOI: 10.1074/jbc.m114.547794
  • Peer Reviewed
• [Presentation] スフィンゴシン１リン酸（S1P）の蛍光標識体を用いた赤血球S1P輸送体の活性測定 (2015)
  • Author(s): 小林直木、大塚正人、山口明人、西毅
  • Organizer: BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸国際展示場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Fluorescence-based rapid measurement of sphingosine 1-phosphate transport activity in rat erythrocytes (2015)
  • Author(s): Naoki Kobayashi, Masato Otsuka, Akihito yamaguchi, Tsuyoshi Nishi
  • Organizer: 14th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases
  • Place of Presentation: The Hungarian Academy of Sciences (Budapest, Hungary)
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] 蛍光標識分子を用いた赤血球スフィンゴシン１リン酸（S1P）輸送体の活性測定 (2015)
  • Author(s): 小林直木、山口明人、西毅
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: デザイン・クリエイティブセンター神戸（兵庫県神戸市）
  • Year and Date: 2015-03-28
• [Presentation] 光反応性アナログを用いたスフィンゴシン１リン酸（S1P）輸送体の探索 (2014)
  • Author(s): 小林直木、山口明人、西毅
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Book] A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes (2017)
  • Author(s): Naoki Kobayashi, Tsuyoshi Nishi
  • Publisher: Springer