| Project/Area Number |
25870706
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
Obstetrics and gynecology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | GBS / 妊婦 / 迅速診断 / 抗体 / B群溶血性連鎖球菌 / 新生児 / B群溶連菌 / モノクローナル抗体 / Streptococcus agalactiae / イムノクロマト |
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| Outline of Final Research Achievements |
Group B Streptococcus (GBS) is a leading cause of serious neonatal infections. CDC recommends GBS screening for all pregnant women. Although GBS screening has been performed mainly by the culture-based method, it takes several days to obtain a reliable result. In this study, we developed an immunochromatographic test (ICT) for the detection of GBS-specific surface immunogenic protein in 15 min from an overnight enrichment culture. This ICT was able to detect recombinant Sip levels 0.5 ng/ml, or about 10＾6 CFU/ml of GBS cells. The cross reactivity test using 26 species of microorganism showed no detectable false positive result. The accuracy of the ICT for GBS detection from enrichment cultures of vaginal swabs was compared with culture-based method. From the results using 260 specimens, the sensitivity and specificity of the ICT appeared 93.1% and 99.6%, respectively. The newly developed ICT is readily applicable to clinical use in the detection of GBS.
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