| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Our aim was to combine radiation therapy with a dose sensitizer to provide sufficient treatment against potential metastasis around a primary lesion. Our potential candidate material was gold nanoparticles (GNPs). We examined the basic requirements for the development of GNPs that would specifically integrate into cancer cells. Breaks in DNA caused by radiation were detected as form changes in the plasmid DNA and were quantified by electrophoresis to evaluate the effect of enhancement of the GNP. We investigated the enhancement effect by changing test conditions, such as the surface charge and particle size of the GNPs. Addition of positively charged GNPs promoted single-strand breaks in the DNA. The GNP effects on radiation-induced DNA damage were influenced by the GNP surface properties and the solution conditions. Particle size, binding method to the target, and blocking agents were important considerations for the development of cancer cell-specific GNPs.
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