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前立腺癌骨転移モデルにおける膜結合型PGES-1の転移抑制機序の解明

Research Project

Project/Area Number 25870709
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Urology
General pharmacology
Research InstitutionKitasato University

Principal Investigator

高橋 亮  北里大学, 医学部, 助教 (30627336)

Project Period (FY) 2013-04-01 – 2014-03-31
Project Status Discontinued (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsprostate cancer / metastasis / angiogenesis
Research Abstract

Prostate cancer is the most commonly malignancy in elderly men in Japan. We have previously reported that host stromal microsomal prostaglandin E synthase-1 (mPGES-1) signaling appeared critical for tumor-associated angiogenesis and tumor growth. We estimated whether mPGES-1 has a critical role in prostate cancer in lung metastasis formation or not.Murine prostate cancer cells (RM9) were intravenously injected to Wild type mice (WT) and mPGES-1 knockout mice (mPGES-1 KO). Lung metastasis was estimated by counting colonies in the lung and the lung weight. Angiogenesis was estimated by measuring plasma level and mRNA expression of angiogenesis stimulates factors, VEGF-A and SDF-1, and microvessel density and microvessel area in the lung.The lung metastasis formation was significantly suppressed in mPGES-1 KO mice compared with WT mice.The number of colonies and the weight of lung is significantly reduced in mPGES-1 KO mice.The plasma and mRNA level of vascular endothelial growth factor (VEGF) and stromal derived factor-1 (SDF-1) were significantly suppressed in mPGES-1 KO mice.The plasma levels of SDF-1 in mPGES-1KO mice were decreased compared with WT.The expressions of VEGF-A and SDF-1 in the lung tissue were significantly suppressed in mPGES-1KO mice. We thought that mPGES-1was important in RM9 hematogenous metastasis to the lungs and in the colonization of tumors after extravasation.The present results indicated that the selective mPGES-1 inhibitors under development will be a promising therapeutic tool for the inhibition of tumor hematogenous metastasis.

Report

(1 results)
  • 2013 Annual Research Report
  • Research Products

    (7 results)

All 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] Roles of microsomal prostaglandin E synthase-1 in lung metastasis formation in prostate cancer RM9 cells.2014

    • Author(s)
      Takahashi R., Amano H., Satoh T., Tabata K., Ikeda M., Kitasato H., Akira S., Iwamura M., Majima M.
    • Journal Title

      Biomed Pharmacother.

      Volume: 68 Issue: 1 Pages: 71-77

    • DOI

      10.1016/j.biopha.2013.10.008

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] マウス前立腺癌の血行性転移モデルにおける膜結合型PGES-1の抗腫瘍効果の検討.

    • Author(s)
      高橋亮、天野英樹、馬嶋正隆
    • Organizer
      第128回日本薬理学会関東部会
    • Place of Presentation
      早稲田大学国際会議場(東京都新宿区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Role of Microsomal prostaglandin E synthase-1 in the facilitation of angiogenesis and lung metastasis formation.

    • Author(s)
      高橋亮、天野英樹、佐藤威文、岩村正嗣、馬嶋正隆
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microsomal prostaglandin E synthase-1 facilitates angiogenesis and lung metastasis formation in prostate cancer cells.

    • Author(s)
      高橋亮、天野英樹、佐藤威文、岩村正嗣、馬嶋正隆
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス前立腺癌の血行性転移モデルにおける膜結合型PGES-1の抗腫瘍効果の検討.

    • Author(s)
      高橋亮、天野英樹、佐藤威文、岩村正嗣、馬嶋正隆
    • Organizer
      第102回日本泌尿器科学会総会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Roles of microsomal prostaglandin E synthase-1 in lung metastasis formation in prostate cancer cells in mice.

    • Author(s)
      Ryo Takahashi, Hideki Amano, Takefumi Satoh, Masatsugu Iwamura, Masataka Majima
    • Organizer
      American Urological Association 109th Annual Meeting
    • Place of Presentation
      Orlando, Florida, United States
    • Related Report
      2013 Annual Research Report
  • [Presentation] Role Of Microsomal Prostaglandin E Synthase-1 Enhances Angiogenesis And Lung Metastasis Formation in Mice.

    • Author(s)
      Ryo Takahashi, Hideki Amano, Takefumi Satoh, Masatsugu Iwamura, Masataka Majima
    • Organizer
      American Thoracic Society 2014 Annual Meeting
    • Place of Presentation
      San Diego, California, United States
    • Related Report
      2013 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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