Enhancement of mechanical properties of stem cell-based self-assembled tissue as a novel medical material for ligament and tendon
Project/Area Number |
25870723
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
Orthopaedic surgery
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Research Institution | Seikei University (2015) Tokai University (2013-2014) |
Principal Investigator |
OYA KEI 成蹊大学, 理工学部, 助教 (50549962)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 幹細胞自己生成組織 / 間葉系幹細胞 / 腱・靭帯 / ナノ周期構造 / フェムト秒レーザ加工 / 動的ひずみ負荷培養 / 静的荷重負荷培養 / 高強度化 / 配向制御 / コラーゲンシート / 静的圧縮荷重下培養 / 動的圧縮荷重下培養 / 一方向磁場刺激 / 複合化 / レーザ加工 / 磁場刺激 / 動的ひずみ負荷 / 超短パルスレーザー加工 / 積層化と複合化 |
Outline of Final Research Achievements |
A stem cell-based self-assembled tissue (scSAT) biosynthesized using synovium-derived mesenchymal stem cells is expected as a novel medical material for ligament and tendon. However, it is necessary to improve the mechanical properties of the scSAT for clinical application. In this study, the scSAT was synthesized on a nanoperiodic structured substrate patterned via a femtosecond laser processing. In addition, dynamic strain and static loading were loaded on the MSCs and their extracellular matrix (ECM) during scSAT synthesis. It was demonstrated that all techniques improved the mechanical properties of the scSAT. The tissue orientation of the scSAT was controlled by the special culturing on a nanoperiodic substrate and loading of dynamic strain. On the other hand, the thickness of the scSAT was thickened by static loading. From these results, it is considered that tissue orientation and enhancement of ECM synthesis contribute to the improvement of mechanical properties of the scSAT.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Stem Cell-Based Self-Assembled Tissues Cultured on a Nano-Periodic-Structured Surface Patterned Using Femtosecond Laser Processing2016
Author(s)
Fujie, H., Oya, K., Tani, Y., Suzuki, K., Nakamura, N.
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Journal Title
International Journal of Automation Technology
Volume: 10
Issue: 1
Pages: 55-61
DOI
NAID
ISSN
1881-7629, 1883-8022
Year and Date
2016-01-05
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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