| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
A stem cell-based self-assembled tissue (scSAT) biosynthesized using synovium-derived mesenchymal stem cells is expected as a novel medical material for ligament and tendon. However, it is necessary to improve the mechanical properties of the scSAT for clinical application. In this study, the scSAT was synthesized on a nanoperiodic structured substrate patterned via a femtosecond laser processing. In addition, dynamic strain and static loading were loaded on the MSCs and their extracellular matrix (ECM) during scSAT synthesis. It was demonstrated that all techniques improved the mechanical properties of the scSAT. The tissue orientation of the scSAT was controlled by the special culturing on a nanoperiodic substrate and loading of dynamic strain. On the other hand, the thickness of the scSAT was thickened by static loading. From these results, it is considered that tissue orientation and enhancement of ECM synthesis contribute to the improvement of mechanical properties of the scSAT.
|
