| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Transcription factor PLZF is involved in various biological processes, including cell proliferation and differentiation. However, the role of PLZF in chondrogenesis is not well understood. In this study, we investigated PLZF expression during chondrocyte differentiation by using a chondrogenic progenitor cell line ATDC5. Our results indicated that PLZF expression was upregulated during chondrocyte differentiation. To elucidate the role of PLZF in chondrogenesis, we transfected ATDC5 cells with the gene encoding PLZF or a shRNA against the gene encoding PLZF. PLZF overexpression suppressed the proliferation and promoted the differentiation of ATDC5 cells into hypertrophic chondrocytes. In contrast, transfection of ATDC5 cells with the shRNA against the gene encoding PLZF suppressed their differentiation into hypertrophic chondrocytes and promoted cell cycle progression. These results suggested that PLZF promoted hypertrophic phenotypes by regulating cell cycle progression.
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