The roles of PLZF transcription factor in osteogenesis and chondrogenesis.

• Project/Area Number: 25870785
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry; General anatomy (including histology/embryology)
• Research Institution: Nihon University
• Principal Investigator: NAITO Masako 日本大学, 歯学部, 准教授 (40436803)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 軟骨細胞分化 / 骨芽細胞分化 / 脂肪細胞分化 / 転写因子 / グルココルチコイド / 軟骨細胞 / glucocorticoid / 細胞周期 / 分化 / 軟骨基質 / CDK inhibitor / 骨芽細胞 / 間葉系前駆細胞 / Glucocorticoid

Outline of Final Research Achievements

Transcription factor PLZF is involved in various biological processes, including cell proliferation and differentiation. However, the role of PLZF in chondrogenesis is not well understood. In this study, we investigated PLZF expression during chondrocyte differentiation by using a chondrogenic progenitor cell line ATDC5. Our results indicated that PLZF expression was upregulated during chondrocyte differentiation. To elucidate the role of PLZF in chondrogenesis, we transfected ATDC5 cells with the gene encoding PLZF or a shRNA against the gene encoding PLZF. PLZF overexpression suppressed the proliferation and promoted the differentiation of ATDC5 cells into hypertrophic chondrocytes. In contrast, transfection of ATDC5 cells with the shRNA against the gene encoding PLZF suppressed their differentiation into hypertrophic chondrocytes and promoted cell cycle progression. These results suggested that PLZF promoted hypertrophic phenotypes by regulating cell cycle progression.

Research Products

• [Journal Article] Promyelocytic leukemia zinc finger mediates glucocorticoid-induced cell cycle arrest in the chondroprogenitor cell line ATDC5. (2015)
  • Author(s): Naito M, Vongsa S, Tsukune N, Ohashi A, Takahashi T.
  • Journal Title: Mol Cell Endocrinol Volume: 417 Pages: 114-123
  • DOI: 10.1016/j.mce.2015.09.026
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dexamethasone inhibits chondrocyte differentiation by suppression of Wnt/β-catenin signaling in the chondrogenic cell line ATDC5. (2015)
  • Author(s): Naito M, Ohashi A, Takahashi T.
  • Journal Title: Histochem Cell Biol. Volume: 144(3) Issue: 3 Pages: 261-272
  • DOI: 10.1007/s00418-015-1334-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3E1 cells. (2015)
  • Author(s): Manaka S, Tanabe N, Kariya T, Naito M, Takayama T, Nagao M, Liu D, Ito K, Maeno M, Suzuki N, Miyazaki M
  • Journal Title: FEBS Letters Volume: 589 Issue: 3 Pages: 310-318
  • DOI: 10.1016/j.febslet.2014.12.013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 軟骨細胞分化におけるLamin A/Cの発現と局在について (2016)
  • Author(s): 内藤昌子、大橋晶子、高橋富久
  • Organizer: 第121回日本解剖学会全国学術集会総会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市）
  • Year and Date: 2016-03-29
• [Presentation] The roles of promyelocytic leukaemia zinc finger (PLZF) in Glucocorticoid-induced cell cycle arrest in a chondrogenic progenitor cells, ATDC5. (2015)
  • Author(s): 内藤昌子 大橋晶子 高橋富久
  • Organizer: 第120日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県・神戸市）
  • Year and Date: 2015-03-22
• [Presentation] 軟骨細胞分化におけるPLZF転写因子の役割について
  • Author(s): 内藤 昌子 高橋 富久
  • Organizer: 第１１９回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学 栃木県