| Project/Area Number |
25870788
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
Applied microbiology
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| Research Institution | Nihon University |
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Principal Investigator |
NARISAWA Naoki 日本大学, 生物資源科学部, 助教 (90632034)
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| Research Collaborator |
TAKENAGA Fumio
TORII Yasuyoshi
ABE Shin
WATANABE Mai
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 魚醤 / 微生物 / バイオフィルム / Staplylococcus / もろみ / 発酵食品 |
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| Outline of Final Research Achievements |
Fish sauce is a liquid extracted from the fermentation of fish with salt. Salt-tolerant bacteria-derived protease may contribute to fermentation of fish sauce. In this study, we investigated biofilm and its function in fish sauce. Fish sauce was prepared by mixing Oncorhynchus mykiss and sterile distilled water with salt. The sample was kept at 37℃ under static condition for 3 months. Modified marine medium was used to isolate bacteria and investigate biofilm mass. We isolated biofilm-forming bacteria from fish sauce mash, and this strain possessed high protease productivity. Results of 16S rRNA gene sequence analysis showed high homology to Streptococcus sp.. Microflora analysis of the fish sauce mash by 16S rRNA clone analysis revealed Streptococcus sp. was dominant species until 3 months after fermentation. Induction of the biofilm into fermentation bath will be of use for an acceleration of fermentation process.
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