| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Giant Vesicle (GV) is a spherical self-assembly with an inner water phase which is separated from a bulk phase by its membrane. GV encapsulation of a chemical reaction which can be triggered by an external stimulus is interesting from the viewpoint of construction of artificial cell containing inter reaction system. In this study, a GV containing a photo-sensitive smaller GV as a microcapsule was constructed by a w/o emulsion centrifugation method using a prepared dispersion of internal microcapsules containing a solution of double stranded DNA (dsDNA): the membrane of the encapsulated microcapsule contains a photo-labile phospholipid. The dsDNA in the microcapsule was released into the water pool of the external GV containing a dsDNA intercalator (SYBR-Green I, SG) by UV irradiation. Photo-induced increase of the fluorescence originated from the dsDNA-SG complex was traced by a real-time fluorescence microscopy.
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