Construction of photo-sensitive GV(giant vesicle)-in-GV inspired by eucaryotic cell structure
| Project/Area Number |
25870837
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional solid state chemistry
Biological physics/Chemical physics/Soft matter physics
|
|---|
| Research Institution | Kanagawa University |
|---|
Principal Investigator |
Suzuki Kentaro 神奈川大学, 理学部, 外部資金雇用研究者 (60512324)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SUGAWARA Tadashi 神奈川大学, 理学部化学科, 教授 (50124219)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | ベシクル / 人工細胞モデル / 光分解反応 / DNA / リン脂質 / 二重ベシクル / ジャイアントベシクル / ケージド化合物 / 紫外線応答性 / 人工細胞 / 光応答型リン脂質 |
|---|
| Outline of Final Research Achievements |
Giant Vesicle (GV) is a spherical self-assembly with an inner water phase which is separated from a bulk phase by its membrane. GV encapsulation of a chemical reaction which can be triggered by an external stimulus is interesting from the viewpoint of construction of artificial cell containing inter reaction system. In this study, a GV containing a photo-sensitive smaller GV as a microcapsule was constructed by a w/o emulsion centrifugation method using a prepared dispersion of internal microcapsules containing a solution of double stranded DNA (dsDNA): the membrane of the encapsulated microcapsule contains a photo-labile phospholipid. The dsDNA in the microcapsule was released into the water pool of the external GV containing a dsDNA intercalator (SYBR-Green I, SG) by UV irradiation. Photo-induced increase of the fluorescence originated from the dsDNA-SG complex was traced by a real-time fluorescence microscopy.
|
Report
(4 results)
Research Products
(54 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Sustainability of Recursive Vesicle-based Protocell2015
Author(s)
Tadashi Sugawara, Kensuke Kurihara, Muneyuki Matsuo, Taro Toyota, Kentaro Suzuki
Organizer
International Symposium on Fluctuation and Structure out of Equilibrium 2015 (SFS2015)
Place of Presentation
Kyoto, Japan
Year and Date
2015-08-20
Related Report
Int'l Joint Research / Invited
-
[Presentation] Construction of Photo-sensitive GV in GV2015
Author(s)
Kentaro Suzuki, Kotaro Machida, Kazuo Yamaguchi, Tadashi Sugawara
Organizer
International Symposium on Fluctuation and Structure out of Equilibrium 2015 (SFS2015)
Place of Presentation
Kyoto, Japan
Year and Date
2015-08-20
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Constructive Approach towards a Vesicle-based Protcell2014
Author(s)
Tadashi Sugawara, Muneyuki Matsuo, Kensuke Kurihara, Taro Toyota, Kentaro Suzuki
Organizer
Origins2014, 2nd ISSOL-The International Astrobiology Society and Bioastronomy (IAU C51) Joint International Conference
Place of Presentation
奈良
Year and Date
2014-07-06 – 2014-07-11
Related Report
-
-
-
-
-
-
-
-
-
-
-
