The identification to transcription factor activated by Akt and the investigation for the relation between the transcription factor and antidepressant effect.
Project/Area Number |
25870844
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Psychiatric science
Medical pharmacy
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Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
Nakano Miho 聖マリアンナ医科大学, 医学部, 助教 (90621574)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | Akt / 抗うつ薬 / BDNF / プロテオミクス / SSRI / フルボキサミン |
Outline of Final Research Achievements |
The expression of brain-derived neurotrophic factor (BDNF) may be a downstream target of a variety of antidepressant treatments, and selective serotonin reuptake inhibitors (SSRIs) are used clinically for the treatment of depression. BDNF binds to and activates tyrosine kinases receptor (TrkB) to exert its effects. TrkB, after activation by ligands, stimulates phosphoinositide 3-kinase (PI3K). This study demonstrates that fluvoxamine treatment rapidly increased phosphorylation of Akt-1. And BDNF activated Akt-1 phosphorylation by the TrkB/ PI3K/Akt-1 pathway. We conclude that the phosphorylation of Akt-1, downstream of PI3K, was the key to their antidepressant effects. We developed the milnaciplan, SNRI, resistant HT22 cell, and detected the changed protein by the proteomics method. We detected some proteins, tRNA ligase, glucose-regulated protein, histone-binding protein compared with control.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] 抗不安薬の適切な使用方法2014
Author(s)
長田賢一, 渡邉高志, 田口篤, 芳賀俊明, 武藤亜矢, 牛谷真由美, 柳田拓洋, 中野三穂, 貴家康男, 山口登.
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Journal Title
Modern Physician
Volume: 34(6)
Pages: 719-723
Related Report
Open Access
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[Presentation] 双極性障害の症状と睡眠障害に対する検討2014
Author(s)
長田賢一, 牛谷真由美, 瀧沢絵里, 中野三穂, 渡邉高志, 田口篤, 芳賀俊明, 武藤亜矢, 貴家康男, 山口登
Organizer
日本睡眠学会第39回定期学術集会
Place of Presentation
あわぎんホール(徳島県・徳島市)
Year and Date
2014-07-03
Related Report
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