Telomere maintenance in quiescent cells
| Project/Area Number |
25870849
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
Genome biology
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | テロメア / ゲノム / チェックポイント / 分裂酵母 / G0細胞 / 分列酵母 / シェルタリン / 増殖停止細胞 |
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| Outline of Final Research Achievements |
The length of telomere DNA of G0 cells of Schizosaccharomyces pombe induced by nitrogen starvation was same as that of logarithmically growing cells. Next, we investigated the amount of the six components of shelterin complex at telomere at G0 cells. The telomere localization of Pot1, Tpz1, and Taz1 of G0 cells was same as that of growing cells. On the other hand, the telomere localization of Rap1, Poz1, and Ccq1 of G0 cells was increased compared to that of growing cells, suggesting that telomere of G0 cells is highly condensed. To evaluate the biological significance of telomere condensation of G0 cells, we constructed a system in which a target protein is specifically eliminated in G0 cells. The viability and telomere DNA length was not affected when each shelterin components was eliminated in G0 cells by using this system. These results suggest that shelterin is not essential for telomere maintenance in G0 cells.
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Report
(4 results)
Research Products
(1 results)
