• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Telomere maintenance in quiescent cells

Research Project

Project/Area Number 25870849
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Genome biology
Research InstitutionNigata University of Phermacy and Applied Life Sciences

Principal Investigator

Yamazaki Harutake  新潟薬科大学, 応用生物科学部, 助教 (20456776)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsテロメア / ゲノム / チェックポイント / 分裂酵母 / G0細胞 / 分列酵母 / シェルタリン / 増殖停止細胞
Outline of Final Research Achievements

The length of telomere DNA of G0 cells of Schizosaccharomyces pombe induced by nitrogen starvation was same as that of logarithmically growing cells. Next, we investigated the amount of the six components of shelterin complex at telomere at G0 cells. The telomere localization of Pot1, Tpz1, and Taz1 of G0 cells was same as that of growing cells. On the other hand, the telomere localization of Rap1, Poz1, and Ccq1 of G0 cells was increased compared to that of growing cells, suggesting that telomere of G0 cells is highly condensed. To evaluate the biological significance of telomere condensation of G0 cells, we constructed a system in which a target protein is specifically eliminated in G0 cells. The viability and telomere DNA length was not affected when each shelterin components was eliminated in G0 cells by using this system. These results suggest that shelterin is not essential for telomere maintenance in G0 cells.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results)

  • [Presentation] 分裂酵母Schizosaccharomyces pombeのテロメア維持におけるシェルタリンの機能解析2015

    • Author(s)
      丸山雅也,山崎晴丈,髙久洋暁
    • Organizer
      「ソイルエンジニアリング」シンポジウム in 長岡
    • Place of Presentation
      新潟県長岡市
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi