The role of intracellular muscarinic M1 receptor in the regulation of synaptic plasticity and spatial memory

• Project/Area Number: 25870852
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology; Neurochemistry/Neuropharmacology
• Research Institution: Kanazawa Medical University
• Principal Investigator: MASUOKA Takayoshi 金沢医科大学, 医学部, 講師 (80509307)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ムスカリン受容体 / シナプス伝達長期増強 / 海馬 / 空間記憶課題 / ムスカリンM1受容体

Outline of Final Research Achievements

Muscarinic acetylcholine receptors are well known to broadly express in brain and contribute to neuropsychological function such as memory and emotion. In present study, we performed experiments to clarify the regulatory mechanism of hippocampal long-term potentiation (LTP) mediated by intracellular M1 receptor expressing in Golgi and endoplasmic reticulum, and the contribution of the intracellular M1 receptor on spatial memory. As a result, it was suggested that intracellular M1 receptor expressing postsynaptic neurons facilitates the efficiency of synaptic transmission on postsynaptic membrane. On the other hand, it was also revealed that LTP facilitation induced by intracellular M1 receptor hardly affect spatial learning.

Research Products

• [Journal Article] Biphasic modulation by mGlu5 receptors of TRPV1-mediatedintracellular calcium elevation in sensory neurons contributes to heat sensitivity. (2015)
  • Author(s): Masuoka T, Nakamura T, Kudo M, Yoshida J, Takaoka Y, Kato N, Ishibashi T,
  • Journal Title: Br J Pharmacol Volume: 172 Issue: 4 Pages: 1020-1033
  • DOI: 10.1111/bph.12962
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Biphasic modulation of noxious heat sensitivity in sensory neurons by peripheral metabotropic glutamate receptors (2015)
  • Author(s): Masuoka T, Ishibashi T. and Nishio M.
  • Journal Title: Inflamm Cell Signal Volume: 2
  • DOI: 10.14800/ics.602
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intracellular localization of M1 muscarinic acetylcholine receptor through clathrin-dependent constitutive internalization via a C-terminal tryptophan-based motif. (2014)
  • Author(s): Uwada J, Yoshiki H, Masuoka T, Nishio M, Muramatsu I.
  • Journal Title: Journal of Cell Science Volume: １２７ Pages: 3131-3140
  • DOI: 10.1242/jcs.148478
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel contribution of cell surface and intracellular M1-muscarinic acetylcholine receptors to synaptic plasticity in hippocampus. (2013)
  • Author(s): Anisuzzaman AS1, Uwada J, Masuoka T, Yoshiki H, Nishio M, Ikegaya Y, Takahashi N, Matsuki N, Fujibayashi Y, Yonekura Y, Momiyama T, Muramatsu I.
  • Journal Title: Journal of Neurochemistry Volume: 126 Issue: 3 Pages: 360-371
  • DOI: 10.1111/jnc.12306
  • Peer Reviewed
• [Presentation] Effects of sodium channel blocker amitriptyline on the spontaneous and stimulus-evoked activity of corneal cold-sensitive nerve terminals in intact and tear-deficit guinea-pig eye (2015)
  • Author(s): Takayoshi Masuoka, Juana Gallar, Carlos Belmonte
  • Organizer: The Association for Research in Vision and Ophtalmology 2015
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2015-05-03 – 2015-05-07
• [Presentation] Resveratrol Downregulated Caveolin-1 in A431 Cells (2015)
  • Author(s): Noriko Imaizumi, Junko Yoshida, Takaharu Ishibashi, Takayoshi Masuoka, and Matomo Nishio
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] Effects of amitriptyline on the spontaneous and stimulus-evoked activity of corneal cold-sensitive nerve terminals (2015)
  • Author(s): Juana Gallar, Takayoshi Masuoka, Carlos Belmonte
  • Organizer: Association for Ocular Pharmacology and Therapeutics 2015
  • Place of Presentation: Charleston, SC, USA
  • Year and Date: 2015-02-26 – 2015-03-01
• [Presentation] Modulation of TRPV1 and voltage-gated calcium channels by peripheral metabotropic glutamate receptors type 5 contributes to heat hyper- and hypoalgesia in mice.
  • Author(s): Takayoshi Masuoka, Tadayuki Nakamura, Junko Yoshida, Yuki Takaoka, Noriko Imaizumi, Takaharu Ishibashi, Matomo Nishio
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego Convention Center, San Diego, USA
• [Presentation] Metabotropic glutamate receptor type 5 biphasically modulates heat pain behavior in mice
  • Author(s): Takayoshi Masuoka, Makiko Kudo, Junko Yoshida, Yuki Takaoka, Takaharu Ishibashi, Matomo Nishio
  • Organizer: 日本薬理学会
  • Place of Presentation: 仙台国際センター（仙台）
• [Presentation] 末梢性代謝型グルタミン酸受容体による痛みの調節機構の解析
  • Author(s): 益岡尚由
  • Organizer: 科学技術振興機構さきがけ研究21、第１４回「知と構成」領域懇話会
  • Place of Presentation: グランドホテル白山（石川）
  • Invited