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Biosynthesis and physiological function of D-glutamate in kuruma prawn Marsupenaeus japonicus

Research Project

Project/Area Number 25870858
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Biomolecular chemistry
Research InstitutionShizuoka Institute of Science and Technology

Principal Investigator

YOSHIKAWA Naoko  静岡理工科大学, 理工学部, 講師 (30392533)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsD-グルタミン酸 / D-アミノ酸 / クルマエビ
Outline of Final Research Achievements

D-Glutamate was specifically detected in male genital tissue of kuruma prawn Marsupenaeus japonicus. To reveal the physiological functions of D-glutamate, the distribution of D-glutamate was examined on these tissues of the prawn. D-Glutamate was found in the testis, vas deferens, and male genital aperture, respectively. The ratio of D-glutamate to total glutamate was over 80% in vas deferens. Moreover, the activity of glutamate racemase which catalyzes the interconversion of D- and L-glutamate was detected in these tissues. Thus, D-glutamate in the prawn is biosynthesized by glutamate racemase.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Effects of high-salinity seawater acclimation on the levels of D-alanine in the muscle and hepatopancreas of kuruma prawn, Marsupenaeus japonicus2015

    • Author(s)
      Naoko Yoshikawa, Masahumi Yokoyama
    • Journal Title

      Journal of Pharmaceutical and Biomedical Analysis

      Volume: in press Pages: 53-58

    • DOI

      10.1016/j.jpba.2015.05.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 甲殻類における遊離型D-アミノ酸の生理機能2014

    • Author(s)
      吉川 尚子
    • Journal Title

      D-アミノ酸学会誌

      Volume: 2 Pages: 7-11

    • Related Report
      2013 Research-status Report
  • [Journal Article] クルマエビにおけるD-アラニンの機能解析2014

    • Author(s)
      吉川 尚子
    • Journal Title

      Journal of the Society of Japanese Women Scientists

      Volume: 14 Pages: 16-20

    • NAID

      130003397221

    • Related Report
      2013 Research-status Report
  • [Presentation] クルマエビ生殖腺におけるD-グルタミン酸の分布2014

    • Author(s)
      吉川 尚子
    • Organizer
      日本生化学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2014-10-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] クルマエビにおける遊離型D-アミノ酸の分布および生理機能

    • Author(s)
      吉川 尚子
    • Organizer
      第9回D-アミノ酸研究会学術講演会
    • Place of Presentation
      関西大学千里山キャンパス100周年記念会館
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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