Application of an enzyme-labeled antigen method for visualizing specific antibody-producing cells in nasal polyp tissue

• Project/Area Number: 25870873
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology; Immunology
• Research Institution: Fujita Health University
• Principal Investigator: ONOUCHI Takanori 藤田保健衛生大学, 医学部, 助教 (20632954)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 酵素抗原法 / 特異抗体産生細胞 / アレルギー性鼻炎 / 鼻腔ポリープ / コナヒョウヒダニ / ヤケヒョウヒダニ / スギ花粉

Outline of Final Research Achievements

The enzyme-labeled antigen method is a novel histochemical technique that visualizes specific antibody-producing cells in tissue sections by employing a biotin-labeled antigen as a probe. The purpose of the present study was to visualize specific antibody-producing cells in the nasal polyp tissue. The plasma cells producing antibodies against the immunized-antigens were visualized in the lymph nodes of rats immunized with Dermatophagoides farina, or Dermatophagoides pteronyssinus, but not in the lymph nodes of rats immunized with Japanese cedar pollen. D. farina, D. pteronyssinus and Japanese cedar pollen reactive plasma cells were not visualized in the nasal polyp tissue.