Visualization of signaling mechanism underlying pre- and postsynaptic functional modification
| Project/Area Number |
25870895
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
General physiology
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | シナプス / シナプス可塑性 / 小脳 / プルキンエ細胞 / 軸索 / 可視化 / 培養 / シナプス前部 / イメージング / 短期シナプス可塑性 / 軸索終末 |
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| Outline of Final Research Achievements |
We developed a method to directly patch-clamp record from the fluorescently labeled axon terminals of cultured Purkinje cells. We found that an axon terminal of a Purkinje cell contains large releasable pool of synaptic vesicles, and that the release probability of each vesicle upon a single action potential is low. In addition, our data clarified a unique presynaptic plasticity mechanism that the low membrane excitability at the axon terminal of a Purkinje cell attenuates action potential amplitudes during high frequency activation, resulting in short-term depression.
We developed a FRET probe monitoring the CaMKII activity mediating the induction of long-term potentiation at inhibitory synapses on a Purkinje cell, which contributes to motor learning. Thus, our probe might enable to real time image the intracellular signaling closely associated with the learning-related plasticity.
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Report
(3 results)
Research Products
(8 results)
