Relationship between the mode change of migrating neuron and vascular network in the developing cerebral cortex

• Project/Area Number: 25870900
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology); General medical chemistry
• Research Institution: Tohoku Medical and Pharmaceutical University (2016); Doshisha University (2013-2015)
• Principal Investigator: NISHIMURA Yoshiaki 東北医科薬科大学, 医学部, 助教 (50508603)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 発生学・形態形成学 / 発生医学

Outline of Final Research Achievements

During brain formation, post-mitotic neurons migrate through the developing vascular network. To analyze their interaction, we first analyzed the mechanisms that control the morphological changes of migrating neurons. By using the ex vivo slice culture system of the cerebral cortex, it became clear that Cdk5, p27kip1, JNK etc. are involved. We also attempted time-lapse observation to analyze the detailed formation process of the vascular network. However, since the vascular network collapsed early in the slice tissue, we failed to record the normal morphological changes and analyze the molecular mechanism.

Research Products

• [Journal Article] Cdk5 and its substrates, Dcx and p27kip1, regulate cytoplasmic dilation formation and nuclear elongation in migrating neurons. (2014)
  • Author(s): Nishimura YV, Shikanai M, Hoshino M, Ohshima T, Nabeshima Y, Mizutani K, Nagata K, Nakajima K and Kawauchi T
  • Journal Title: Development Volume: 141 Issue: 18 Pages: 3540-3550
  • DOI: 10.1242/dev.111294
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] SIL1, a causative cochaperone gene of Marinesco-Sjogren syndrome, plays an essential role in establishing the architecture of the developing cerebral cortex. (2014)
  • Author(s): Hamada N, Inaguma Y, Tabata H, Iwamoto I, Mizuno M, Nishimura YV, Ito H, Morishita R, Suzuki M, Ohno K, Kumagai T, Nagata K.
  • Journal Title: EMBO Mol Med. Volume: 6 Issue: 3 Pages: 414-29
  • DOI: 10.1002/emmm.201303069
  • Peer Reviewed / Open Access
• [Presentation] 大脳皮質形成の制御機構の研究 -神経細胞移動と血管網形成の関係- (2017)
  • Author(s): 西村嘉晃
  • Organizer: 第3回日本血管生物医学会若手研究会
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2017-03-03
• [Presentation] 胎生期における脳血管網のパターン形成 (2016)
  • Author(s): 西村嘉晃
  • Organizer: 第2回血管生物医学会若手研究会
  • Place of Presentation: 東北大学加齢医学研究所
  • Year and Date: 2016-03-04
• [Presentation] 発生期大脳皮質における神経細胞移動の形態変化を制御する機構の解析 (2015)
  • Author(s): 西村嘉晃
  • Organizer: 第42回脳神経科学コアセンターセミナー
  • Place of Presentation: 東北大学医学系研究科
  • Year and Date: 2015-12-18
  • Invited
• [Presentation] 正常な脳内環境構築における血管網形成と神経組織形成 (2015)
  • Author(s): 西村嘉晃
  • Organizer: 第1回日本血管生物医学会若手研究会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-02-06 – 2015-02-07
• [Presentation] 発生期大脳皮質におけるロコモーション移動の形態変化を制御する機構の解析 (2015)
  • Author(s): 西村嘉晃
  • Organizer: 第2回包括的神経グリア研究会(UNG2015)
  • Place of Presentation: オースプラザ（名古屋）
  • Year and Date: 2015-01-10 – 2015-01-11
• [Presentation] 発生期のマウス大脳皮質において Cdk5とJNKは異なる様式で 神経細胞に特徴的な移動パターンを決定する (2014)
  • Author(s): 西村嘉晃、鹿内弥磨、星野幹雄、大島登志男、鍋島陽一、水谷健一、永田浩一、仲嶋一範、川内健史
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Cdk5は移動神経細胞に特異的な"dilation"の形成と核の形態変化を制御する (2014)
  • Author(s): 西村嘉晃、鹿内弥磨、星野幹雄、大島登志男、鍋島陽一、水谷健一、永田浩一、仲嶋一範、川内健史
  • Organizer: 第36回日本生物学的精神医学会・第57回日本神経化学会大会合同年会
  • Place of Presentation: 奈良県分化会館
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] SIL1, a causative gene of Marinesco-Sjogren syndrome, plays an essential role in establishing the architecture of the developing cerebral cortex (2013)
  • Author(s): Kohichi Nagata, Yutaka Inaguma, Nanako Hamada, Hidenori Tabata, Yoshiaki Nishimura, Hidenori Ito, Makoto Mizuno, Ikuko Iwamoto, Rika Morishita, Motomasa Suzuki, Toshiyuki Kumagai
  • Organizer: 第36回日本神経科学大会、第56回日本神経化学会大会、第23回日本神経回路学会大会
  • Place of Presentation: 京都国際会館
• [Presentation] マウス発生期大脳皮質の血管網形成 (2013)
  • Author(s): Yoshiaki V. Nishimura, Yutaka Inaguma, Masatsugu Ema, Mitsuharu Hattori, Koh-ichi Nagata, Ken-ichi Mizutani
  • Organizer: 第21回日本血管生物医学会学術集会
  • Place of Presentation: 大阪千里阪急ホテル