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Systematic mechanisms for regeneration of central nervous system via the cerebrospinal fluid

Research Project

Project/Area Number 25870950
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nerve anatomy/Neuropathology
Emergency medicine
Research InstitutionAino University

Principal Investigator

HOMMA Tamami  藍野大学, 再生医療研究所, 研究員 (30631220)

Research Collaborator IDE Chizuka  藍野大学, 医療保健学部, 教授 (70010080)
NAKANO Norihiko  藍野大学, 医療保健学部, 准教授 (40322721)
MATSUMOTO Naoya  大阪大学, 救急医学教室, 助教 (50359808)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords脈絡叢 / 骨髄間質細胞 / 神経再生 / 脊髄損傷 / 外傷外科学 / 神経可塑性
Outline of Final Research Achievements

The expression of mRNAs of NGF, VEGF, HGF, IGF-1, and FGF-2 was up-regulated in CPECs under the co-culture with BMSCs, but, there was no significant difference. The co-culture with BMSCs in the medium containing LPS showed that mRNAs of IGF-1 and FGF-2 increased significantly. Next, the real-time PCR showed that the mRNA of NGF was up-regulated in the co-cultures with BMSCs, while that of HGF was increased only when co-cultured with mono-layered BMSCs. There was no change with VEGF. The protein expressions of NGF were up-regulated in individual cultures of BMSCs and CPECs in the LPS-containing medium. The co-culture showed that NGF of CPECs was up-regulated to the level higher than the total values of the individual cultures, indicating that NGF was enhanced by the co-culture. Expressions of VEGF remained at the same level as the total values of individual cultures of BMSCs and CPECs, meaning that there was no enhancement of VEGF production by the co-culture.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (9 results)

  • [Journal Article] Are the long-term survival, proliferation, and differentiation of transplanted cells desirable in clinical application for spinal cord injury?2016

    • Author(s)
      Kanekiyo K, Nakano N, Homma T, Yamada Y, Tamachi M, Ohta M, Suzuki Y, Ide C
    • Journal Title

      Aino Journal

      Volume: 14

    • NAID

      110010060827

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Locomotor improvement of spinal cord-injured rats through body weight support treadmill training by plantar placement of hind paws on the floor.2015

    • Author(s)
      Hayashibe M, Homma T, Fujimoto K, Oi T, Yagi N, Kashihara M, Nishikawa N, Ishizumi Y, Abe S, Hashimoto H, Kanekiyo K, Ide C, Morioka S
    • Journal Title

      Spinal Cord

      Volume: 186

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of bone marrow stromal cell transplantation through CSF on the subacute and chronic spinal cord injury in rats2013

    • Author(s)
      Nakano N, Nakai Y, Seo TB, Homma T, Yamada Y, Ohta M, Suzuki Y, Nakatani T, Fukushima M, Hayashibe M, Ide C
    • Journal Title

      PloS ONE

      Volume: 8(9) Issue: 9 Pages: e73494-e73494

    • DOI

      10.1371/journal.pone.0073494

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Transplantation of choroid plexus epithelial cells into contusion-injured spinal cord of rats2016

    • Author(s)
      兼清健志、中野法彦、本間玲実、野田亨、井出千束
    • Organizer
      第121回日本解剖学会総会全国学術集会
    • Place of Presentation
      福島
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脊髄損傷モデルラットにおける脈絡叢上皮細胞による神経再生効果2016

    • Author(s)
      兼清健志、中野法彦、本間玲実、野田亨、井出千束
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脊髄損傷ラットへの脈絡叢上皮細胞移植効果2015

    • Author(s)
      兼清健志、中野法彦、本間玲実、野田亨、井出千束
    • Organizer
      第56回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 骨髄間質細胞により発現が誘導される脈絡叢上皮細胞由来の神経再生因子の同定2014

    • Author(s)
      兼清健志
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] A Search for Nerve Regeneration Factors from Choroid Plexus Epithelial Cells2014

    • Author(s)
      兼清健志
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-01 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] ラット骨髄間質細胞培養上清の投与による神経再生に対する効果2014

    • Author(s)
      中野法彦、兼清健志、本間玲実、井出千束
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 骨髄間質細胞により発現が誘導される脈絡叢上皮細胞由来の神経再生因子の同定2014

    • Author(s)
      兼清健志、本間玲実、中野法彦、松本直也、井出千束
    • Organizer
      第119回日本解剖学会総会全国学術集会
    • Place of Presentation
      栃木
    • Related Report
      2013 Research-status Report
  • [Presentation] 骨髄間質細胞の培養メディウムによる脊髄損傷ラットに対する行動回復効果2013

    • Author(s)
      本間玲実、中野法彦、井出千束
    • Organizer
      第36回日本神経科学大会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] ラット骨髄間質細胞培養上清による神経再生の解析2013

    • Author(s)
      中野法彦、本間玲実、兼清健志、井出千束
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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