| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Phosphorylation of high mobility group box 1 (HMGB1) is involved in the subcellular translocation of the protein and subsequent secretion. Phosphorylation of serine residues in the two nuclear localization signals (NLS1 and NLS2) promotes nucleocytoplasmic relocation of HMGB1. In this study, it was suggested that protein phosphatase 2A (PP2A) correlates in the nucleocytoplasmic shuttling of HMGB1 through dephosphorylation of specific phosphoserines within NLS1. Next, kinases that phosphorylate serine residues within NLS1 were predicted, and an in vitro kinase assay was performed. Among the predicted kinases, protein kinase C phosphorylated Ser46 of HMGB1-derived peptides, and a mutagenesis experiment implied that the phosphorylation could induce translocation of HMGB1 to the cytosol.
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