Elucidation of phosphorylation mechanism participate in nucreocytoplasmic shuttling and secretion of HMGB1
Project/Area Number |
25870990
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Biomolecular chemistry
Functional biochemistry
|
Research Institution | Kyushu Institute of Technology (2014-2015) Kurume University (2013) |
Principal Investigator |
Taira Junichi 九州工業大学, 大学院情報工学研究院, 助教 (20549612)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | HMGB1 / PP2A / 核移行シグナル / PKC / GSK-3 / CK2 / NLS / RAW264.7 |
Outline of Final Research Achievements |
Phosphorylation of high mobility group box 1 (HMGB1) is involved in the subcellular translocation of the protein and subsequent secretion. Phosphorylation of serine residues in the two nuclear localization signals (NLS1 and NLS2) promotes nucleocytoplasmic relocation of HMGB1. In this study, it was suggested that protein phosphatase 2A (PP2A) correlates in the nucleocytoplasmic shuttling of HMGB1 through dephosphorylation of specific phosphoserines within NLS1. Next, kinases that phosphorylate serine residues within NLS1 were predicted, and an in vitro kinase assay was performed. Among the predicted kinases, protein kinase C phosphorylated Ser46 of HMGB1-derived peptides, and a mutagenesis experiment implied that the phosphorylation could induce translocation of HMGB1 to the cytosol.
|
Report
(4 results)
Research Products
(24 results)
-
-
[Journal Article] Improvement of heme oxygenase-1-based heme sensor for quantifying free heme in biological samples2015
Author(s)
Junichi Taira, Yukinori Nakashima, Shun Yoshihara, Shinya Koga, Shinji Sueda, Hideyuki Komatsu, Yuichiro Higashimoto, Toru Takahashi, Nohito Tanioka, Hiroko Shimizu, Hiroshi Morimatsu, Hiroshi Sakamoto
-
Journal Title
Analytical Biochemistry
Volume: 489
Pages: 50-52
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
[Journal Article] Blockade by phosphorothioate aptamers of advanced glycation end products-induced damage in cultured pericytes and endothelial cells2013
Author(s)
Higashimoto, Y., Matsui, T., Nishino, Y., Taira, J., Inoue, H., Takeuchi, M., Yamagishi, S.I.
-
Journal Title
Microvasc. Res.
Volume: 90
Pages: 64-70
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Tyr誘導体の細胞毒性2013
Author(s)
平 順一, 上田敏久, 宗 伸明, 関 清彦, 光富 勝
Organizer
2013年度日本農芸化学会関西・中四国・西日本支部合同大会
Place of Presentation
県立広島大学広島キャンパス(広島市)
Related Report
-
-