Characteristics and biological function of anti-pMHC antibody

• Project/Area Number: 25870992
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery; Immunology
• Research Institution: Kurume University
• Principal Investigator: KOMATSU Nobukazu 久留米大学, 医学部, 講師 (50343687)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 抗体 / ペプチド・MHC複合体 / がんワクチン / pMHC

Outline of Final Research Achievements

I have demonstrated surface plasmon resonance analysis for an antibody recognized pMHC complex in human peripheral blood. Antibody was detected, albeit low affinity and low binding levels. Then, IgG fraction was purified from plasma sample by protein G column chromatography. During CTL stimulation assay, IFN-gamma production was increased in PBMC cultured with peptide, IgG fraction, although it is not significant statistically. However IgG fraction is not effective cytotoxicity mediated by ADCC. Therefore, an antibody recognized pMHC complex is not seem to be main cytotoxic mechanism during peptide vaccination therapy.

Research Products

• [Journal Article] Humoral immune responses to CTL epitope peptides from tumor-associated antigens are widely detectable in humans: A new biomarker for overall survival of patients with malignant diseases (2013)
  • Author(s): Matsueda, S., Komatsu, N., Kusumoto, K., Koga, S., Yamada, A., Kuromatsu, R., Yamada, S., Seki, R., Yutani, S., Shichijo, S., Mine, T., Fukuda, T., Okamura, T., Okuda, S., Sata, M., Honda, J., Kaji, M., Itoh, K., Sasada, T.
  • Journal Title: Developmental and Comparative Immunology Volume: 41 (1) Pages: 68-76
  • Peer Reviewed
• [Journal Article] Fine-mapping naturally occurring NY-ESO-1 antibody epitopes in melanoma patients' sera using short overlapping peptides and full-length recombinant protein (2013)
  • Author(s): Komatsu, N., Jackson, H.M., Chan, K.-F., Oveissi, S., Cebon, J., Itoh, K., Chen, W.
  • Journal Title: Molecular Immunology Volume: 54 (3-4) Pages: 465-471
  • Peer Reviewed
• [Journal Article] Personalized Peptide Vaccine as a Novel Immunotherapy Against Advanced Cancer (2013)
  • Author(s): Komatsu, N., Matsueda, S., Noguchi, M., Yamada, A., Noguchi, M., Itoh, K., Sasada, T.
  • Journal Title: Molecular Vaccines Volume: Part IV Pages: 361-369
• [Presentation] Recent advances of peptide-based cancer vaccine (2014)
  • Author(s): Itoh K, Noguchi M, Yamada A, Shichijo S, Sasada T, Matsueda S, Komatsu N.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25 – 2014-09-27