Research Project
Grant-in-Aid for Young Scientists (B)
I have demonstrated surface plasmon resonance analysis for an antibody recognized pMHC complex in human peripheral blood. Antibody was detected, albeit low affinity and low binding levels. Then, IgG fraction was purified from plasma sample by protein G column chromatography. During CTL stimulation assay, IFN-gamma production was increased in PBMC cultured with peptide, IgG fraction, although it is not significant statistically. However IgG fraction is not effective cytotoxicity mediated by ADCC. Therefore, an antibody recognized pMHC complex is not seem to be main cytotoxic mechanism during peptide vaccination therapy.
All 2014 2013
All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (1 results)
Developmental and Comparative Immunology
Volume: 41 (1) Pages: 68-76
Molecular Immunology
Volume: 54 (3-4) Pages: 465-471
Molecular Vaccines
Volume: Part IV Pages: 361-369