| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
A novel and selective extraction method for nucleotides based on non-covalent fluorous biphasic extraction system in the presence of perfluoroalkylamine as an ion-pair reagent. The ion-pairs between nucleotides and perfluoroalkylamine were selectively extracted to a fluorous solvent via the affinity of perfluoroalkyl-containing compounds with each other, i.e. fluorous interaction. Then, the nucleotides in the fluorous solvent could be back-extracted with aqueous ammonia in acetonitrile as non-fluorous solvent, and the extracts were directly injected to LC system. A hydrophilic interaction chromatography was utilized for separation of the nucleotides by gradient elution with the mixture of acetonitrile, water, and ammonium carbonate buffer as mobile phase. This method was successfully applied to the determination of nucleotides in some cell samples.
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