STAT3 mediated acquisition of stem cell features in glioblastoma cells

Research Project

Project/Area Number	25871006
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Tumor biology Pathological medical chemistry
Research Institution	Kyorin University
Principal Investigator	CHIBA Tomohiro 杏林大学, 医学部, 助教 (60398617)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	癌幹細胞 / 神経膠芽腫 / STAT3 / リプログラミング / グリオブラストーマ / micro RNA
Outline of Final Research Achievements	Cancer stem cells (CSC) is implicated in the resistance to chemotherapy. We hypothesized that CSC could be induced by cellular stress such as chemotherapeutic agents through activation of STAT3. We observed high levels of phosphorylated STAT3 in human glioblastoma (GBM) samples together with high levels of stem cell markers such as Sox2, 5-lipoxygenase etc. Stem cell markers are induced by cellular stress, which can be regulated by endogenous micro RNA-302/367 cluster. These findings provide a novel insight into CSC-targeted therapy for GBM.