| Project/Area Number |
25871040
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
Applied microbiology
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| Research Institution | Wakayama National College of Technology |
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Principal Investigator |
Kusube Masataka 和歌山工業高等専門学校, 物質工学科, 准教授 (40403761)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 食品加工 / 微高圧炭酸ガス殺菌 / 高圧力 / 細胞内酸性化 / 微高圧炭酸ガス / 圧力 / 殺菌 / 不活化 / 微生物 / 加圧 / 高圧pH測定 / 微高圧 / 炭酸ガス |
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| Outline of Final Research Achievements |
The petit-high pressure carbon dioxide gas for microbe sterilization was suggested in this study. Carbon dioxide dissociates to carbonic acid (H2CO3) and second dissociates to proton and carbonate ion (HCO3-). These dissociates are promoted by pressurizing cause these reaction volume change shows negative value, i.e., this technique is very useful under high pressure. Saccharomyces cerevisiae S288C culturing cells were diluted to 104-105 by YPB fresh media, Tris Based Saline (TBS) buffer and 0.85% distilled saline (NaCl) and was CO2 pressurized until 1.0 MPa at 30 or 40 °C for each hours. Inactivation rate (kd) was decided from colony forming unit (CFU). The kd value of NaCl diluted yeast cells is remarkable increased compared with other diluted solutions. We decided temperature and proton are most effective factors for yeast inactivation by statistical analysis and multiple linear regression analysis.
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