Diversity of thalamic relay neurons projecting to mouse primary visual cortex

• Project/Area Number: 25871060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology; Basic / Social brain science
• Research Institution: Shinshu University (2015); National Institute for Physiological Sciences (2013-2014)
• Principal Investigator: MORI Takuma 信州大学, 学術研究院医学系, 助教 (70545798)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 狂犬病ウイルス / 神経回路トレース / 大脳新皮質 / 機能性神経回路 / 越シナプストレース / 視覚

Outline of Final Research Achievements

I analyzed the distribution of presynaptic cells to layer 2/3 pyramidal cells of mouse neocortex using rabies monosynaptic tracing. Rabies virus is various among a group of various experimental strains. I used rabies glycoproteins originated from two different strains of rabies virus for the monosynaptic tracing. I found that monosynaptic tracing using different strains of rabies glycoprotein visualized distinct types of cells in mouse brain. An avirulent rabies glycoprotein helped rabies virus to infect not only neurons but also glial cells around the injection site. Considering that some glial cells, such as astrocytes and microglia, contribute to the immune system, these results suggest that infection of rabies virus to glial cells may decrease the virulency of rabies virus.

Research Products

• [Journal Article] IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) interacts with gephyrin to promote inhibitory synapse formation (2016)
  • Author(s): Ji Won Um, Gayoung Choii, Dongseok Park, Dongwook Kim, Sangmin Jeon, Hyeyeon Kang, Takuma Mori, Theofilos Papadopoulos, Taesun Yoo, Yeunkum Lee, Eunjoon Kim, Katsuhiko Tabuchi and Jaewon Ko
  • Journal Title: The Journal of Biological Chemistry Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Rabies virus glycoprotein variants display different patterns in rabies monosynaptic tracing. (2014)
  • Author(s): Takuma Mori, Kinjiro Morimoto
  • Journal Title: Frontiers in Neuroanatomy Volume: 7 Pages: 47-47
  • DOI: 10.3389/fnana.2013.00047
  • Peer Reviewed
• [Presentation] Retrograde monosynaptic tracing with a recombinant rabies virus reveals transient monosynaptic connections between the corticospinal and motor neurons during development in mice (2015)
  • Author(s): Naoyuki Murabe, Satoshi Fukuda, Noriko Isoo, Takuma Mori, Hiroaki Mizukami, Keiya Ozawa, Yumiko Yoshimura and Masaki Sakurai
  • Organizer: 北米神経科学会
  • Place of Presentation: McCormick Place、シカゴ、米国
  • Year and Date: 2015-10-17
• [Presentation] 発達過程における皮質脊髄―運動ニューロン単シナプス結合の除去：改変狂犬病ウイルスを用いた逆行性単シナプストレーシングによる研究 (2015)
  • Author(s): 村部直之、福田諭、磯尾紀子、森琢磨、水上浩明、小澤敬也、吉村由美子、桜井正樹
  • Organizer: 日本神経科学学会
  • Place of Presentation: 神戸市、神戸国際会議場
  • Year and Date: 2015-07-28
• [Presentation] マウス海馬 CA1 における錐体細胞および介在細胞サブタイプへの異なったシナプス入力様式 (2014)
  • Author(s): 森琢磨、吉村由美子
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 神奈川県横浜市
  • Year and Date: 2014-09-13
• [Presentation] 幼若マウス皮質には運動ニューロンと直接結合する皮質脊髄神経細胞が広範に分布する:糖タンパク 質欠損狂犬病ウイルスを用いた単シナプス逆行性トレーシング (2014)
  • Author(s): 村部 直之,福田 諭,森 琢磨,水上 浩明,小澤 敬也,吉村 由美子,桜井 正樹
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 神奈川県横浜市
  • Year and Date: 2014-09-13
• [Book] 脳神経外科医が知っておくべきニューロサイエンスの知識 (2015)
  • Author(s): 森 琢磨
  • Total Pages: 236
  • Publisher: 文光堂