| Project/Area Number |
25871075
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
Tumor therapeutics
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
Katano Ikumi 公益財団法人実験動物中央研究所, その他部局等, 研究員 (90442558)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ヒト化マウス / NK細胞 / ヒト免疫 / ｈIL-2 / hIL-15 / hIL-2 |
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| Outline of Final Research Achievements |
We previously reported that human NK cells are predominately developed in NOG-hIL-2 Tg mice after human hematopoietic stem cell (HSC) transplantation. In this study, we assessed in vivo antibody-dependent-cellular-cytotoxicity (ADCC) activity using human HSC-transferred NOG-hIL-2 Tg mice engrafted with CCR4 expressing Hodgkin’s lymphoma cell line L428. After treatment with an anti-CCR4 antibody, tumor growth was slightly suppressed in NOG-hIL-2 Tg mice. To improve the function of NK cells, we generated a NOG-hIL-15 Tg mouse. Although human mature NK cells were dominantly differentiated in NOG-hIL-15 Tg mice, they immediately died within 8 weeks after HSC transplantation. In contrast, human peripheral blood-derived mature NK cells succeeded to be maintained over 3 months after transplantation without death. Moreover, these NK cells showed in vivo cytotoxic activity against engrafted K562 cells. Therefore, NOG-hIL-15 Tg mice are useful tools for investigation of in vivo NK cytotoxicity.
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