Structure basis for E2-E3 interation in plant Atg conjugation system

• Project/Area Number: 25871076
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry; Functional biochemistry
• Research Institution: Microbial Chemistry Research Foundation
• Principal Investigator: MATOBA Kazuaki 公益財団法人微生物化学研究会, 微生物化学研究所, 研究員 (60613792)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: X線結晶構造解析 / オートファジー

Outline of Final Research Achievements

We identified the minimum binding region of Atg3 for Atg12 by in vitro pull-down assay using Arabidopsis thaliana (At) homologs and crystallized the AtAtg12b-AtAtg3 peptide complex. The side-chain of AtAtg3 Asp-Met is bound deeply to the pocket of AtAtg12b. The importance of AtAtg3 Met157 for AtAtg12b binding was confirmed by mutational analysis. These data establish the basis for E2-E3 interaction in the plant Atg system.

Research Products

• [Journal Article] Atg12-Atg5 conjugate enhances E2 activity of Atg3 by rearranging its catalytic site (2013)
  • Author(s): Sakoh-Nakatogawa, M., Matoba, K., Asai, E., Kirisako, H., Ishii, J., Noda, N. N., Inagaki, F., Nakatogawa, H. and Ohsumi, Y
  • Journal Title: Nature Structural & Molecular Biology Volume: Vol.20 Issue: 4 Pages: 433-439
  • DOI: 10.1038/nsmb.2527
  • Peer Reviewed
• [Presentation] Structure basis for E2-E3 interaction in the plant Atg conjugation system (2014)
  • Author(s): Kazuaki Matoba, Yuko Fujioka, Machiko Sakoh-Nakatogawa, Hitoshi Nakatogawa, Yoshinori Ohsumi, Nobuo N. Noda
  • Organizer: 23rd Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Palais des congres de Montreal (Canada)
  • Year and Date: 2014-08-07
• [Remarks] 微生物化学研究所
  • URL: http://www.bikaken.or.jp/