Development of cesium-accumulating mutant of radioresistant bacteria by ion beam breeding technology
| Project/Area Number |
25871092
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
Quantum beam science
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
SATOH Katsuya 独立行政法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究副主幹 (90370402)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 微生物バイオレメディエーション技術 / 放射線抵抗性細菌 / イオンビーム突然変異育種技術 / 放射性セシウム / デイノコッカス・ラジオデュランス / 環境修復 / 環境保全 / 突然変異株 / イオンビーム突然変異育種 / デイノコッカス属細菌 / セシウム蓄積能 |
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| Outline of Final Research Achievements |
We conducted the development of high Cs-accumulating mutant from Deinococcus radiodurans using ion beam breeding technology. D. radiodurans were mutagenized with ion beams that were the highest mutant frequency observed (1E-1 to 1E-2 of surviving-fraction). The imaging analysis using Cs-137 was performed to estimate the Cs-accumulating ability of the mutagenized cells. Candidates of Cs-accumulating mutant were screened on the basis of the luminescent intensity and the cell area as indicators of intracellular Cs level and cell growth, respectively. As a result, we obtained 33 candidates, whose Cs-accumulating abilities were over 2-fold higher than that of the wild-type, from the population of mutagenized cells (about 3,000). Although more detailed analysis regarding the Cs-accumulating ability and the sensitivity to gamma rays are needed, these candidates generated by ion beam breeding technology could be expected to use for bioremediation purpose.
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Report
(3 results)
Research Products
(12 results)
