| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Forage digestibility decreases with secondary wall deposition during stem development. To analyze transcriptional network regulating secondary wall biosynthesis in forage grasses, suppression subtractive hybridization was used to identify differentially expressed genes between wild-type tall fescue and transgenic one expressing OsSWN2 chimeric repressor that caused a reduction of cell wall thickening in sclerenchyma cells. After differential screening, differentially expressed genes were categorized into various functional categories according to their putative functions by BLAST analysis. Two transcription factors, FaMYB and FaNAC, were isolated as candidate genes involved in secondary wall biosynthesis. To characterize their function, transgenic tall fescue was generated by using CRES-T.
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