Effect of Neurokinin B on the activity of the GnRH pulse generator

• Project/Area Number: 25871109
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science; Animal production science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: Wakabayashi Yoshihiro 国立研究開発法人農業生物資源研究所, 動物生産生理機能研究ユニット, 主任研究員 (00510695)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: キスペプチン / neurokinin B / 弓状核 / ダイノルフィン / ニューロキニン / ニューロキニンB

Outline of Final Research Achievements

Neurokinin B (NKB) in the arcuate nucleus (ARC) kisspeptin neurons might regulate the pulsatile secretion of GnRH/luteinizing hormone (LH) in mammals, however physiological function of NKB still remains unclear. To verify the physiological function of NKB, goats bearing the guide cannula with recording electrodes in the ARC, where kisspeptin neurons are located, were received micro injection of NKB. The local injection of NKB evoked bursts of the ARC kisspeptin neurons and following secretion of LH. These findings suggest that NKB in the ARC kisspeptin neurons stimulates the neural activity of the ARC kisspeptin neurons to burst simultaneously. This burst might be neural source of the pulsatile secretion of GnRH/LH.

Research Products

• [Journal Article] Effects of intravenous administration of neurokinin receptor subtype-selective agonists on gonadotropin-releasing hormone pulse generator activity and luteinizing hormone secretion in goats (2015)
  • Author(s): Yamamura T, Wakabayashi Y, Ohkura S, Navarro VM, Okamura H
  • Journal Title: Journal of Reproduction and Development Volume: 61 Issue: 1 Pages: 20-29
  • DOI: 10.1262/jrd.2014-109
  • NAID: 130004700243
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Identification of an olfactory signal molecule that activates the central regulator of reproduction in goats (2014)
  • Author(s): Murata K., Tamogami S., Itou M., Ohkubo Y., Wakabayashi Y., Watahabe H., Okamura H., Takeuchi Y., Mori Y.
  • Journal Title: Curr Biol Volume: 24 Issue: 6 Pages: 681-686
  • DOI: 10.1016/j.cub.2014.01.073
  • Peer Reviewed
• [Journal Article] Ovarian and hormonal responses to follicular phase administration of investigational metastin/kisspeptin analog, TAK-683, in goats. (2014)
  • Author(s): Goto, Y., Endo, N., Nagai, K., Ohkura, S., Wakabayashi, Y., Tanaka, A., Matsui, H., Kusaka, M., Okamura, H. and Tanaka, T.
  • Journal Title: Reproduction in Domestic Animals Volume: 49 Issue: 2 Pages: 338-342
  • DOI: 10.1111/rda.12283
  • Peer Reviewed
• [Presentation] 弓状核へのNeurokinin B局所投与により誘起された神経活動上昇は反対側の弓状核キスペプチンニューロン群へ伝達される (2015)
  • Author(s): 若林嘉浩、山村崇、大蔵聡、岡村裕昭
  • Organizer: 第108回 日本繁殖生物学会大会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2015-09-16
• [Presentation] シバヤギ弓状核キスペプチンニューロン分布領域へのNeurokinin B局所投与がGnRHパルス産生におよぼす影響 (2014)
  • Author(s): 若林嘉浩、山村崇、大蔵聡、岡村裕昭
  • Organizer: 第107回 日本繁殖生物学会
  • Place of Presentation: 帯広畜産
  • Year and Date: 2014-08-20 – 2014-08-23
• [Presentation] シバヤギをモデルとした視床下部による繁殖制御機構の研究 (2014)
  • Author(s): 若林嘉浩
  • Organizer: 日本実験動物科学技術さっぽろ2014
  • Place of Presentation: 札幌
  • Invited
• [Presentation] 新規κオピオイド受容体拮抗剤の末梢投与がシバヤギGnRHパルスジェネレーター活動に及ぼす効果 (2013)
  • Author(s): 伊藤太佑、中務桂佑、若林嘉浩、山村崇、岡村裕昭、大石真也、野口太郎、藤井信孝、上野山賀久、束村博子、前多敬一郎、松田二子、大蔵聡
  • Organizer: 第106回 日本繁殖生物学会大会
  • Place of Presentation: 東京
• [Book] Advances in Experimental Medicine and Biology (2013)
  • Author(s): Okamura H., Tsukamura H., Ohkura S., Uenoyama Y., Wakabayashi Y., Maeda K-I.
  • Total Pages: 27
  • Publisher: Kisspeptin and GnRH pulse generation. In: Kisspeptin Signaling in Reproductive Biology.