| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We developed a comprehensive system for generation and evaluation of iPS cells and generated functional rat germ cells from rat iPS cells in mouse-rat interspecific chimeras.
Additionally, we generated a novel pluripotent stem cells named INTPS cells by culturing ES cells in EpiSC medium containing a GSK3 inhibitor. The INTPS cells have intermediate feature between ES cells and EpiSCs and have an ability to contribute to chimeras and their germline.
We proposed that this culture condition is useful for generating novel pluripotent stem cells in non-rodent species, because this culture condition enables us to establish pluripotent stem cells independently from LIF signaling.
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