| Project/Area Number |
25871118
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
Medical systems
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| Research Institution | Tokyo University of Science (2014-2015)
Institute of Physical and Chemical Research (2013) |
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Principal Investigator |
Ishihara Ryo 東京理科大学, 基礎工学部, 助教 (30633507)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 表面機能化自律駆動マイクロチップ / 放射線グラフト重合法 / マイクロRNA / バイオマーカー / その場診断 / microRNA検出 / 自律駆動マイクロチップ / 放射線グラフト重合 / 高感度 / 短時間 |
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| Outline of Final Research Achievements |
Toward cancer point-of-care (POC) diagnosis, a graft-type power-free microchip for microRNA, cancer biomarker, detection was prepared by radiation beam-induced graft polymerization (RIGP). The prepared portable microchip enables rapid and highly sensitive miRNA detection from small sample volume. Because RIGP is applicable to various polymers without initiator immobilization nor long irradiation time, RIGP is easy and rapid polymer surface modification method. Three dimensional high density capture probe DNA immobilization onto the microchannel inner surface of the microchip was achieved. Limit of detection of microRNA was 0.75 pM, which is same detection sensitivity as a commercially available standard microarray. The required sample volume was 0.5 microliter and assay time was 20 min. The microchip will contribute as a promising platform for POC cancer diagnosis.
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