• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

PtdIns(3,4,5)P3 suppresses PTEN membrane binding in spontaneous formation of cellular polarity

Research Project

Project/Area Number 25871120
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MATSUOKA Satomi  独立行政法人理化学研究所, 生命システム研究センター, 研究員 (00569733)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords細胞極性 / 1分子イメージング / PTEN / PtdIns(3,4,5)P3 / 自己組織化 / 細胞性粘菌 / PI(3,4,5)P3
Outline of Final Research Achievements

PtdIns(3,4,5)P3-enriched domain on cell membrane directs cell migration. I have examined single-molecule behaviors of 3-phosphatase, PTEN, in living Dictyostelium discoideum cells. PTEN undergoes transitions among 3 states with different lateral diffusivities. Enzymatically active state is the moderate one. Dephosphorylation accompanies prompt membrane dissociation. An amount of the active state depends on transition from the slowest state. PtdIns(3,4,5)P3 suppresses this state, finally reducing the PTEN amount on the membrane. Thus, PtdIns(3,4,5)P3 excludes PTEN from the membrane, illustrating that the positive feedback causes spontaneous formation of the domain in random cell migration.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] PTEN hopping on the cell membrane is regulated via a positively-charged C2 domain.2014

    • Author(s)
      Yasui M, Matsuoka S and Ueda M
    • Journal Title

      PLoS Computational Biology.

      Volume: 10 Issue: 9 Pages: e1003817-e1003817

    • DOI

      10.1371/journal.pcbi.1003817

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 1分子イメージングによる走化性情報伝達系の動態解析2014

    • Author(s)
      松岡里実、上田昌宏
    • Journal Title

      生体の科学

      Volume: 65 Pages: 394-395

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Intracellular Encoding of Spatiotemporal Guidance Cues in a Self-Organizing Signaling System for Chemotaxis in Dictyostelium Cells.2013

    • Author(s)
      Shibata T, Nishikawa M, Matsuoka S, Ueda M
    • Journal Title

      Biophys J.

      Volume: 105(9) Issue: 9 Pages: 2199-2209

    • DOI

      10.1016/j.bpj.2013.09.024

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] PTENの1分子イメージングによる細胞極性形成メカニズムの解析2015

    • Author(s)
      松岡里実,上田昌宏
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      京都、京都国際会館
    • Year and Date
      2015-05-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Single-Molecule Imaging Analysis of PI3K and PTEN in Establishing PIP3 Polarity2015

    • Author(s)
      松岡里実,福島誠也、上田昌宏
    • Organizer
      Gordon Research Conference: Directed Cell Migration
    • Place of Presentation
      Galveston, TX, USA
    • Year and Date
      2015-01-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] PI(3,4,5)P3-induced PTEN membrane release provides a positive feedback mechanism for cell polarity2014

    • Author(s)
      松岡里実,上田昌宏
    • Organizer
      39th Symposium on Hormones and Cell Regulation
    • Place of Presentation
      Mont Sainte-Odile, Alsace, France
    • Year and Date
      2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Single-molecule imaging anlaysis of PI3K in chemotactic cell2014

    • Author(s)
      福島誠也、松岡里実,上田昌宏
    • Organizer
      39th Symposium on Hormones and Cell Regulation
    • Place of Presentation
      Mont Sainte-Odile, Alsace, France
    • Year and Date
      2014-10-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of the Regulation Mechanism of PI3K Activity by Live-cell Single-molecule Imaging2014

    • Author(s)
      福島誠也、松岡里実,上田昌宏
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌、札幌コンベンションセンター
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] PTEN Membrane Binding is Destabilized by PI(3,4,5)P3: Positive Feedback Loop in Establishing Cellular Polarity2014

    • Author(s)
      松岡里実,上田昌宏
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌、札幌コンベンションセンター
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] PTENの極性形成メカニズムの1分子イメージング解析

    • Author(s)
      松岡里実,柴田達夫,上田昌宏
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      大阪,ホテル阪急エキスポパーク
    • Related Report
      2013 Research-status Report
  • [Presentation] Positive feedback mechanism for PIP3 polarity establishment mediated by PIP3 phosphatase, PTEN

    • Author(s)
      松岡里実,上田昌宏
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都,国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Book] Chemotaxis:javascript:onSave() Methods and Protocols, Second Edition2015

    • Author(s)
      Satomi Matsuoka, Yukihiro Miyanaga, Masahiro Ueda
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] 1分子生物学2014

    • Author(s)
      松岡里実、上田昌宏
    • Total Pages
      292
    • Publisher
      化学同人
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi