| Project/Area Number |
25871121
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
YUTAKA Ogawa 独立行政法人理化学研究所, 今本細胞核機能研究室, 研究員 (70624956)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 遺伝子発現 / mRNA / １分子感度 |
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| Outline of Final Research Achievements |
In order to visualize specific mRNA and its translated products simultaneously at a single molecular level in eukaryotic cells, we developed a new microscopy with single molecule sensitivity, and established various cell lines of Saccharomyces cerevisiae and human, which stably express reporter genes. Because Saccharomyces cerevisiae exhibited strong autofluorescence in the cytoplasm, we used plasma membrane localization reporter genes. In human cell lines, we used a microtubule-binding reporter gene. In both cases, we succeeded in visualizing specific reporter proteins in a single molecule sensitivity. In addition to reporter proteins, we also tried to visualize its mRNA in a single molecule sensitivity.
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