Regulation of myoblast differentiation through the endoplasmic reticulum (ER) membrane dynamics caused by ER stress

• Project/Area Number: 25871124
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology; General anatomy (including histology/embryology)
• Research Institution: Nara Institute of Science and Technology (2016); Institute of Physical and Chemical Research (2013-2015)
• Principal Investigator: Nakanishi Keiko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (30415252)
• Research Collaborator: MORISHIMA NOBUHIRO 国立研究開発法人理化学研究所, 伊藤ナノ医工学研究室, 専任研究員 (40182232)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞分化 / 小胞体ストレス / オルガネラ動態 / 筋芽細胞 / カルシウム / STIM1 / 筋発生 / 小胞体膜動態

Outline of Final Research Achievements

We previously reported that endoplasmic reticulum (ER) stress signaling caused physiologically during myoblast differentiation plays important roles for the differentiation. However, the mechanism which causes the ER stress signaling was unknown. We investigated correlation between ER membrane dynamics and alterations in ER homeostasis, and revealed that ER calcium depletion is the cause of ER stress during myotube formation.

Research Products

• [Journal Article] Proplatelet formation from megakaryocytes is associated with endoplasmic reticulum stress (2016)
  • Author(s): Nobuhiro Morishima and Keiko Nakanishi
  • Journal Title: Genes to Cells Volume: 21 Issue: 7 Pages: 798-806
  • DOI: 10.1111/gtc.12384
  • Peer Reviewed / Open Access
• [Journal Article] Transient ca2+ depletion from the endoplasmic reticulum is critical for skeletal myoblast differentiation (2015)
  • Author(s): K. Nakanishi, K. Kakiguchi, S. Yonemura, A. Nakano, and N. Morishima
  • Journal Title: The FASEB Journal Volume: 29 Issue: 5 Pages: 2137-2149
  • DOI: 10.1096/fj.14-261529
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Significance of ER Ca2+ outflow during myogenesis (2015)
  • Author(s): N. Morishima and K. Nakanishi
  • Journal Title: Channels Volume: 9 Issue: 4 Pages: 173-174
  • DOI: 10.1080/19336950.2015.1069504
  • Open Access
• [Presentation] 筋分化過程における生理的な小胞体ストレスが引き起こす特異的な小胞体構造変化 (2014)
  • Author(s): 中西慶子、垣口貴沙、米村重信、小林秀俊、中野明彦、森島信裕
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂（奈良）
  • Year and Date: 2014-06-11 – 2014-06-13
• [Remarks] 小胞体内カルシウムの減少に依存した筋分化制御機構
  • URL: http://biomedcircus.com/paper_03_39.html
• [Remarks] 筋肉動かすCaは筋肉作る指令役も担う
  • URL: http://scienceportal.jst.go.jp/news/newsflash_review/newsflash/2015/02/20150218_03.html#
• [Remarks] 筋肉を動かすカルシウムは筋肉を作る指令役も担う
  • URL: http://www.riken.jp/pr/press/2015/20150217_1/
• [Remarks] SARC bodies: markers of cellular stress
  • URL: http://www.riken.jp/en/pr/topics/2015/20150226_1/