Development of real-time detection method of mRNA dynamics for the study of signal regulation system during left-right asymmetry formation
| Project/Area Number |
25871128
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
Developmental biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TAKAI Akira 独立行政法人理化学研究所, 生命システム研究センター, 特別研究員 (60637205)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | シグナル伝達 / 発光イメージング / RNAイメージング / ライブイメージング / ルシフェラーゼ |
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| Outline of Final Research Achievements |
In this research, we developed brighter, cyan and orange variants of Nano-lantern, a recently developed super-brilliant yellowish-green luminescent protein which is suitable for in vivo imaging. These multicolor Nano-lanterns enable monitoring of multiple biological events, including dynamics of intracellular microstructures, gene expressions, and calcium ion concentrations. Furthermore, we developed real-time mRNA detection method by combining Nano-lantern with RNA binding proteins. After further improvement of this mRNA detection method, we will apply it in a study of signal regulation mechanisms during left-right asymmetry formation.
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Report
(3 results)
Research Products
(7 results)
