Role and localization of Polycomb-mediated histone H2A ubiquitination
| Project/Area Number |
25871129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
ENDOH Mitsuhiro 独立行政法人理化学研究所, 統合生命医科学研究センター, 客員研究員 (40391883)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | エピジェネティクス / ヒストン修飾 / クロマチン / 転写制御 / 遺伝子発現 / 胚性幹細胞 / 転写 / 発生 / 分化 / 減数分裂 |
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| Outline of Final Research Achievements |
It is still unclear how Polycomb group proteins Ring1A/B, E3 ubiquitin ligases for histone H2A, recognize and bind to their target genes. In this study, we focused on Polycomb Repressive Complex 1 (PRC1) and MBLR complex, both of with include Ring1A/B. We found that the physical interaction of Ring1B with chromo-domain proteins Cbx2/7, which have an affinity for PRC2-mediated trimethylated histone H3-K27, is essential for PRC1 recruitment to the developmental regulator genes. On the other hand, MBLR complexes recognize and bind to genes related to meiosis dependently on transcription factors Max/Mga, and repress those target genes through Ring1A/B-mediated H2A monoubiquitination.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Gene regulation. Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells2015
Author(s)
Arner E, Daub CO, Vitting-Seerup K, Andersson R, Lilje B, Drabløs F, Lennartsson A, Rönnerblad M, Hrydziuszko O, Vitezic M, Freeman TC, Alhendi AM, Arner P, Axton R, Baillie JK, Beckhouse A, Bodega B, Briggs J, Brombacher F, Davis M, Detmar M, Ehrlund A, Endoh M, Eslami A, Fagiolini M, et al.
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Journal Title
Science
Volume: 347
Issue: 6225
Pages: 1010-1014
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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