| Project/Area Number |
25871130
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
Tumor therapeutics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TADA Seiichi 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 基礎科学特別研究員 (30598165)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 葉酸 / 葉酸レセプターβ / ペプチドアプタマー / in vitroセレクション / リボソームディスプレイ / cDNAディスプレイ / 非天然アミノ酸 / ドラッグデリバリー / 急性骨髄性白血病 |
|---|
| Outline of Final Research Achievements |
In vitro selection method of peptide aptamers binding to only beta type of folate receptor, without alpha type, was investigated. A folic acid molecule was introduced the random peptide library sequence for better binding affinity of selected peptides. The specificity of the selected peptide against the folate receptor types was provided by evolutionary molecular engineering method. Folic acid-conjugating aminoacyl tRNA, which is necessary to incorporate folic acid molecule into the random peptide library, was successfully synthesized. The peptide selection methods were investigated for the removal of remained free folic acid molecules from translated products. Based on these investigation, folic acid-conjugating peptide aptamers binding to folate receptor beta is expected to be selected soon.
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