Development of a data processing system for LC-QqQ/MS based metabolomics
| Project/Area Number |
25871136
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Life / Health / Medical informatics
Biomolecular chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TSUGAWA Hiroshi 独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (30647235)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | メタボロミクス / データ処理 / 化合物同定 / 質量分析 / LC/QqQ/MS / ワイドターゲット解析 / データ解析 / バイオインフォマティクス / 液体クロマトグラフィー |
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| Outline of Final Research Achievements |
We developed a data processing pipeline for widely targeted metabolomics by using the multiple reaction monitoring (MRM) mode. Widely targeted metabolomics became increasingly popular in metabolomics for the simultaneous analysis of several hundred metabolites at high sensitivity and selectivity. However, the identification criterion to distinguish the target metabolites from the isomeric metabolites or background noise has not been developed. In addition, a user-friendly software to deal with the large-scale data sets is required. MRMPROBS was therefore developed for the assessment of large-scale MRM data applicable to any instrument or experimental conditions.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Data Assessment and Metabolite Identification Tool for Large-scale MRM-based Widely Targeted Metabolomics2013
Author(s)
Tsugawa, H., Arita, M., Kanazawa, M., Ogiwara, A., Bamba, T., Fukusaki, E
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Journal Title
Anal. Chem
Volume: 85(10)
Issue: 10
Pages: 5191-5199
DOI
Related Report
Peer Reviewed
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