| Project/Area Number |
25871137
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | National Institute of Technology, Kumamoto College (2014)
Institute of Physical and Chemical Research (2013) |
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Principal Investigator |
TOMIZAWA Satoshi 熊本高等専門学校, 生物化学システム工学科, 助教 (90634709)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | バイオマス / PHA / PHB / リグニン / ポリヒドロキシブタン酸 / 芳香族カルボン酸 / R. eutropha H16 |
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| Outline of Final Research Achievements |
To investigate the conversion of lignin derivatives to bio-polymers containing aromatic monomers, polyhydroxyalkanoate (PHA)-accumulating strains were cultured on mineral salt media containing each of the lignin derivatives and hydroxybenzoic acids, including intermediates derived from the metabolism of lignin derivatives in bacteria. Ralstonia eutropha H16 could synthesize PHA from hydroxybenzoic acids, but aromatic polymers were not produced in this strain. To examine the ability of PHA synthase, I tried in vitro polymerization of aromatic polymers using wild-type PHA synthases and aromatic monomers. However, aromatic polymers were not synthesized in this study.
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