| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
The use of light as an external stimulus offers the potential for spatiotemporal control and is thus ideal for controlling protein expression in living cells. We have developed a reversible method for regulating protein expression using a photoresponsive-cap that can control the translation of mRNA in a reversible manner by triggering the cis-trans photoisomerization of the cap. We succeeded in controlling the amount, timing and duration of protein expression in living mammalian cells. Additionally, neuronal differentiation of PC12 cells was photo-induced by controlling constitutively active H-Ras 61L protein expression.
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