Development of the method for controlling stem cell fate

Research Project

Project/Area Number	25871140
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Biomedical engineering/Biomaterial science and engineering Developmental biology
Research Institution	Hokkaido University
Principal Investigator	OGASAWARA Shinzi 北海道大学, 創成研究機構, 特任助教 (50462669)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	光遺伝学 / mRNA / 翻訳 / 細胞分化 / 再生医療 / タンパク質 / 遺伝子発現
Outline of Final Research Achievements	The use of light as an external stimulus offers the potential for spatiotemporal control and is thus ideal for controlling protein expression in living cells. We have developed a reversible method for regulating protein expression using a photoresponsive-cap that can control the translation of mRNA in a reversible manner by triggering the cis-trans photoisomerization of the cap. We succeeded in controlling the amount, timing and duration of protein expression in living mammalian cells. Additionally, neuronal differentiation of PC12 cells was photo-induced by controlling constitutively active H-Ras 61L protein expression.