| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
This decade, using feeder cells has been standard in the culture of iPS cells. However, the condition of feeder cells as an environmental factor directly gives the change to quality of iPS cells. Therefore, I tried to find the new effective-condition for the culture of mouse-iPS cells without feeder cells.
The iPS cells, iPS-MEF-Ng-20D-17, were incubated on the soft substrates, and I found that the soft substrate was effective in the culture. The effect was enhanced by addition of two inhibitors, GSK3 inhibitor and MAP kinase inhibitor. Second, I tried to investigate the mechanism for maintaining of the iPS cells in this culture condition. In my current study by microarray analysis, the key molecules for maintaining of the iPS cells on the soft substrate, is becoming clearer. The culture condition without special substrate, such as feeder and soft substrate, is under developing based on the result of microarray analysis.
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