| Project/Area Number |
25871145
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
Structural biochemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Nobuhisa Umeki 国立研究開発法人理化学研究所, 佐甲細胞情報研究室, 研究員 (70647502)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | Ras / RalGDS / 1分子計測 / 細胞内情報伝達 / 構造多型 / 一分子計測 / 低分子量Gタンパク質 / 低分子量G蛋白質 |
|---|
| Outline of Final Research Achievements |
To understand the mechanism of Ras-RalGDS-Ral signaling, translocation dynamics of RalGDS and its functional domains (RBD and REMCDC) to the plasma membranes of living cells were measured. Although the RBD played an important role in increasing the association rate constant between RalGDS and the plasma membrane, the REMCDC domain affected the dissociation rate constant from the membrane, which decreased after Ras activation. Thus, multiple regions and domains of both Ras and RalGDS work concertedly to regulate the Ras-RalGDS-Ral signaling. It is also suggested that the structural polymorphism of Ras is involved in interaction of Ras and RalGDS.
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