| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
S-palmitoylation is a reversible post-translational modification that can control the surface expression, spatial organization, interactions, and functional activities of proteins. However, little is known about whether and how TRP channels are regulated by S-palmitoylation. We investigated the role of S-palmitoylation in the in vitro and in vivo activity of several TRP channels. Treatment with a palmitoyl acyltransferase (PAT) enzyme inhibitor 2-bromopalmitate (2BP) altered the agonist-induced responses of TRPM8, TRPA1, and TRPV2 channels in HEK293T cells. Meanwhile, co-expression of TRP channels with the global PAT enzyme DHHC7, but not DHHC3, significantly increased the responses of TRPM8 and TRPA1. Moreover, treatment with 2BP and N-tert-butyl-hydroxylamine (NtBHA), a chemically cleaving reagent of thioester linkage, decreased the responses and percentages in a population of endogenous TRPM8 and TRPA1. Thus, expression of some TRP channels are regulated by S-palmitoylation.
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