Possible Immune Regulation of Natural Killer T Cells in a Model of Metal Ion-Induced Allergic Contact Dermatitis.

• Project/Area Number: 25871220
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology; Surgical dentistry
• Research Institution: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
• Principal Investigator: Gotoh Akito 独立行政法人国立病院機構(相模原病院臨床研究センター), 臨床免疫研究室, 研究員 (50518131)
• Research Collaborator: SUZUKI Ryuji 独立行政法人国立病院機構(相模原病院臨床研究センター), 臨床免疫研究室, 室長 (70373470)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 金属アレルギー / TCR解析 / NKT / 口腔内粘膜疾患 / Ⅳ型アレルギー / TCR / IV型アレルギー / 自己免疫疾患

Outline of Final Research Achievements

Metal often causes delayed-type hypersensitivity reactions, which are possibly mediated by accumulating T cells in the inflamed skin, called irritant or allergic contact dermatitis. However, accumulating T cells during development of a metal allergy are poorly characterized because a suitable animal model is unavailable. We have previously established novel murine models of metal allergy and found accumulation of both metal-specific T cells and natural killer (NK) T cells in the inflamed skin. In our novel models of metal allergy, skin hypersensitivity responses were induced through repeated sensitizations by administration of metal chloride and lipopolysaccharide into the mouse groin followed by metal chloride challenge in the footpad. These models enabled us to investigate the precise mechanisms of the immune responses of metal allergy in the inflamed skin. We consider the immune regulation of accumulated NK T cells in metal ion-induced allergic contact dermatitis.

Research Products

• [Journal Article] Possible Immune Regulation of Natural Killer T Cells in a Murine Model of Metal Ion-Induced Allergic Contact Dermatitis. (2016)
  • Author(s): Kumagai K, Horikawa T, Shigematsu H, Matsubara R, Kitaura K, Eguchi T, Kobayashi H, Nakasone Y, Sato K, Yamada H, Suzuki S, Hamada Y, Suzuki R.
  • Journal Title: International journal of molecular sciences Volume: 12 Issue: 1 Pages: 87-87
  • DOI: 10.3390/ijms17010087
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] NKG2D+ IFN-γ+ CD8+ T cells are responsible for palladium allergy. (2014)
  • Author(s): Kawano M, Nakayama M, Aoshima Y, Nakamura K, Ono M, Nishiya T, Nakamura S, Takeda Y, Dobashi A, Takahashi A, Endo M, Ito A, Ueda K, Sato N, Higuchi S, Kondo T, Hashimoto S, Watanabe M, Watanabe M, Takahashi T, Sasaki K, Nakamura M, Sasazuki T, Narushima T, Suzuki R, Ogasawara K.
  • Journal Title: PLoS One. Volume: 9 Issue: 2 Pages: 1-12
  • DOI: 10.1371/journal.pone.0086810
  • Peer Reviewed / Open Access
• [Journal Article] NKG2D functions as an activating receptor on natural killer cells in common marmoset (callithrix jacchus). (2014)
  • Author(s): Watanabe M, Kudo Y, Kawano M, Nakayama M, Nakamura K, Kameda M, Ebara M, Sato T, Nakamura M, Omine K, Kametani Y, Suzuki R, Ogasawara K.
  • Journal Title: Int Immunol. Volume: 26 Issue: 11 Pages: 597-606
  • DOI: 10.1093/intimm/dxu053
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of T cell receptors of Th1 cells infiltrating inflamed skin of a novel murine model of palladium-induced metal allergy. (2014)
  • Author(s): Shigematsu H, Kumagai K, Kobayashi H, Eguchi T, Kitaura K, Suzuki S, Horikawa T, Matsutani T, Ogasawara K, Hamada Y, Suzuki R.
  • Journal Title: PLoS One. Volume: 9(1) Issue: 1 Pages: e85983-e85983
  • DOI: 10.1371/journal.pone.0085983
  • Peer Reviewed
• [Journal Article] Characterization of T cell receptors of Th1 cells infiltrating inflamed skin of a novel murine model of palladium-induced metal allergy. (2013)
  • Author(s): Kobayashi H, Kumagai K, Eguchi T, Shigematsu H, Kitaura K, Kawano M, Horikawa T, Suzuki S, Matsutani T, Ogasawara K, Hamada Y, Suzuki R.
  • Journal Title: PLoS One Volume: 8 Issue: 10 Pages: e76385-e76385
  • DOI: 10.1371/journal.pone.0076385
  • Peer Reviewed
• [Journal Article] Accumulation of invariant NKT cells into inflamed skin in a novel murine model of nickel allergy. (2013)
  • Author(s): Eguchi T, Kumagai K, Kobayashi H, Shigematsu H, Kitaura K, Suzuki S, Horikawa T, Hamada Y, Ogasawara K, Suzuki R.
  • Journal Title: Cell Immunol. Volume: 284(1-2) Issue: 1-2 Pages: 163-71
  • DOI: 10.1016/j.cellimm.2013.07.010
  • Peer Reviewed