The elucidation of inflammatory response mechanism in the hyperoxic condition during extracorporeal circulation

• Project/Area Number: 25871231
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery; General surgery
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: FUJII Yutaka 独立行政法人国立循環器病研究センター, 研究所, 特任研究員 (20589303)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 体外循環 / 高酸素 / 活性酸素 / 炎症反応 / サイトカイン / 補助循環 / 人工心肺 / 活性酸素種

Outline of Final Research Achievements

Systemic inflammatory responses in patients receiving cardiac surgery supported by cardiopulmonary bypass (CPB) significantly contribute to CPB-associated morbidity and mortality. We hypothesized that hyperoxia insufflation aggravates the inflammatory responses and organ damage during CPB. To verify this hypothesis, we investigated the inflammatory responses at high and normal levels of arterial pressure of oxygen (PaO2) in the rat CPB model.
In this study suggest that it is better to avoid too much oxygen insufflation for
attenuating organ damage associated with the superoxide production and inflammatory responses during CPB.

Research Products

• [Journal Article] A novel small animal extracorporeal circulation model for studying pathophysiology of cardiopulmonary bypass. (2015)
  • Author(s): Fujii Y, Shirai M, Inamori S, Takewa Y, Tatsumi E.
  • Journal Title: J Artif Organs Volume: 18 Issue: 1 Pages: 35-39
  • DOI: 10.1007/s10047-014-0804-y
  • Peer Reviewed
• [Journal Article] Investigation of the biological effects of artificial perfusion using rat extracorporeal circulation model. (2014)
  • Author(s): Fujii Y, Shirai M, Inamori S, Takewa Y, Tatsumi E.
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc Volume: S Pages: 4483-6
  • DOI: 10.1109/embc.2014.6944619
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Acute Rho-kinase inhibition improves coronary dysfunction in vivo, in th e early diabetic microcirculation. (2013)
  • Author(s): Pearson JT, Jenkins Mi. Edgley AJ, Sonobe T, Joshi M. Waddingham MT, Fujii V. Schwenkc DO, Tsuchi mochi H. Yoshimoto M, Umetani K, Kelly DJ, Shirai M.
  • Journal Title: Cardiovasc Diabetol Volume: 12 Issue: 1 Pages: 111-124
  • DOI: 10.1186/1475-2840-12-111
  • Peer Reviewed
• [Journal Article] The Serotonin Pathway-A Therapeutic Target For Pulmonary Hypertension? Assessing Vascular Function Using Synchrotoron Radiation Microangiography. (2013)
  • Author(s): Gray EA, Tsuchimochi H, Pearson JT, Sonobe T, Fujii Y, Yoshimoto M, Umetani K, Shirai M, Schwenke DO.
  • Journal Title: J Synchrotron Radiat Volume: 20(5) Issue: 5 Pages: 756-764
  • DOI: 10.1107/s0909049513021213
  • Peer Reviewed
• [Journal Article] Hyperoxic condition promotes an inflammatory response during cardiopulmonary bypass in a rat model. (2013)
  • Author(s): Fujii Y, Shirai M, Tsuchimochi H, Pearson JT, Takewa Y, Tatsumi E, Taenaka Y.
  • Journal Title: Artif Organs. Volume: 37(12) Issue: 12 Pages: 1034-40
  • DOI: 10.1111/aor.12125
  • Peer Reviewed
• [Journal Article] A comparative study of cerebral microcirculation during pulsatile and nonpulsatile selective cerebral perfusion: Assessment by synchrotron radiation microangiography. (2013)
  • Author(s): Inamori S, Shirai M, Yahagi N, Pearson JT, Fujii Y, Umetani K, Kobayashi Y, Okura Y, Yakehiro M, Minamiyama M.
  • Journal Title: ASAIO J Volume: 59 Issue: 4 Pages: 374-379
  • DOI: 10.1097/mat.0b013e3182976939
  • Peer Reviewed
• [Presentation] Development of new leukocyte removal column aimed at suppression of the inflammatory response during cardiopulmonary bypass ‐Biological evaluation in a rat model‐ (2015)
  • Author(s): Fujii Y, Shirai M, Takewa Y, Tatsumi E
  • Organizer: The 92th Annual Meeting of the physiological Society of Japanese
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-22
• [Presentation] 体外循環中の炎症反応抑制を目指した白血球除去カラムの開発 ─ラット補助循環モデルを用いた検討─ (2015)
  • Author(s): 藤井 豊，白井 幹康，武輪 能明，巽 英介
  • Organizer: 第42回日本集中治療学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-02-09
• [Presentation] 補助循環中の高酸素管理が生体に及ぼす影響‐小動物補助循環モデルを用いた検討- (2014)
  • Author(s): 藤井豊, 白井幹康, 武輪能明, 巽英介
  • Organizer: 第52回日本人工臓器学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2014-10-18
• [Presentation] The effect of blood contact surface area reduction during cardiopulmonary bypass in a rat model (2014)
  • Author(s): Fujii Y, Shirai M, Takewa Y, Tatsumi E.
  • Organizer: 41st Annual ESAO Congress
  • Place of Presentation: Roma, Itary
  • Year and Date: 2014-09-20
• [Presentation] Investigation of the Biological Effects of Artificial Perfusion Using Rat Extracorporeal Circulation Model (2014)
  • Author(s): Fujii Y, Shirai M, Inamori S, Takewa Y, Tatsumi E.
  • Organizer: 36th Annual International IEEE EMBS
  • Place of Presentation: Chicago, USA
  • Year and Date: 2014-08-29
• [Presentation] High Oxygen Partial Pressure Promotes an Inflammatory Response During Extracorporeal Circulation in a Rat Model (2014)
  • Author(s): Fujii Y, Shirai M, Takewa Y, Taenaka Y, Tatsumi E.
  • Organizer: ASAIO 60th Annual Conference
  • Place of Presentation: Washington.D.C. USA
  • Year and Date: 2014-06-19
• [Presentation] The effect of blood contact surface area during cardiopulmonary bypass –Biological body evaluation in a rat model-
  • Author(s): Yutaka Fujii, Mikiyasu Shirai, Yoshiaki Takewa, Eisuke Tatsumi
  • Organizer: The 91th Annual Meeting of the physiological Society of Japanese
  • Place of Presentation: 鹿児島市
• [Presentation] Hyperoxia condition Promotes an inflammatory response during extracorporeal circulation in rat model
  • Author(s): Yutaka Fujii, Eisuke Tatsumi, Mikiyasu Shirai, Yoshiaki Takewa, Yoshiyuki Taenaka
  • Organizer: 51st Annual Meeting of Japanese Society of Artificial Organs (JSAO2013)/The 5th Congress of the International Federation for Artificial Organs (IFAO2013)
  • Place of Presentation: 横浜市
• [Presentation] Hydrogen gas suppresses systemic inflammatory response during cardiopulmonary bypass in a rat model
  • Author(s): Yutaka Fujii, Mikiyasu Shirai, Yoshiaki Takewa, Eisuke Tatsumi, Yoshiyuki Taenaka
  • Organizer: ASAIO 59th Annual Conference
  • Place of Presentation: Chicago, USA
• [Presentation] 低侵襲体外循環を目指した低充填量回路の作製 -ラット体外循環モデルを用いた評価-
  • Author(s): 藤井豊，白井幹康，武輪能明，巽英介，妙中義之
  • Organizer: 第36回日本バイオレオロジー学会
  • Place of Presentation: 福岡市