Analyses of vascular lumen formation by in vivo imaging

• Project/Area Number: 25871232
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology; Morphology/Structure
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: HIROYUKI Nakajima 独立行政法人国立循環器病研究センター, 研究所, 研究員 (10467657)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 血管形成 / シアストレス / 管腔形成 / 4Dイメージング

Outline of Final Research Achievements

We constructed transgenic zebrafish lines that can label apical membranes and cell-cell junctions specifically in endothelial cells. By using these lines, we have revealed that some processes of lumen formation are driven by blood flow. In addition, we found that YAP1, a target of the Hippo pathway, translocated into the nucleus just after lumen formation. Finally, we have demonstrated that YAP1 is regulated by blood flow after lumen formation.

Research Products

• [Journal Article] β-Catenin-dependent transcription is central to Bmp-mediated formation of venous vessels. (2015)
  • Author(s): Kashiwada T, Fukuhara S (Co-corresponding author), Terai K, Tanaka T, Wakayama Y, Ando K, Nakajima H, Fukui H, Yuge S, Saito Y, Gemma A, Mochizuki N.
  • Journal Title: Development Volume: 142 Pages: 497-509
  • DOI: 10.1242/dev.115576
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recent advances in understanding of cardiovascular development by imaging of zebrafish. (2015)
  • Author(s): Fukuhara S, Fukui H, Wakayama Y, Ando K, Nakajima H, Mochizuki N.
  • Journal Title: Dev Growth Differ. Volume: 未定 Issue: 4 Pages: 109-122
  • DOI: 10.1111/dgd.12210
  • Peer Reviewed / Open Access
• [Journal Article] S1P-Yap1 signaling regulates endoderm formation required for cardiac precursor cell migration in zebrafish. (2014)
  • Author(s): Fukui H, Terai K, Nakajima H, Chiba A, Fukuhara S, Mochizuki N.
  • Journal Title: Dev. Cell. Volume: 31 Issue: 1 Pages: 128-136
  • DOI: 10.1016/j.devcel.2014.08.014
  • Peer Reviewed
• [Journal Article] Identification of Inter-Organ Vascular Network: Vessels Bridging between Organs (2013)
  • Author(s): Omae, M, et. al.
  • Journal Title: PLOS ONE Volume: 8 Issue: 6 Pages: e65720-e65720
  • DOI: 10.1371/journal.pone.0065720
  • Peer Reviewed
• [Presentation] 中嶋洋行、山本希美子、福井一、福原茂朋、望月直樹 (2015)
  • Author(s): 血流による機械的刺激に対するYAP1の応答機構
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール船堀（東京）
  • Year and Date: 2015-06-30 – 2015-07-02
• [Presentation] 血流による機械的刺激に対する内皮細胞の新たな応答機構の解析 (2014)
  • Author(s): 中嶋洋行、福井一、福原茂朋、望月直樹
  • Organizer: 第5回Molecular Cardiovascular Conference II
  • Place of Presentation: 神戸ベイシェラトンホテル＆タワーズ（神戸）
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] In vivo imaging of YAP dynamics in zebrafish vascular development (2014)
  • Author(s): Hiroyuki Nakajima, Sigetomo Fukuhara, Naoki Mochizuki
  • Organizer: The 18th International Vascular Biology Meeting （IVBM2014）
  • Place of Presentation: みやこめっせ京都市勧業館（京都）
  • Year and Date: 2014-04-14 – 2014-04-17